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Protocols

• PEG Purification of PCR Products (GSCWU)
  This protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing
  http://genome.wustl.edu/tools/protocols/index.php?...
  Added: Tue May 14 2002, Hits: 682, Reviews: 0  Write review   Cached
• Purification and Sequencing of PCR Product (Donis-Keller lab)
  This method is used to sequence directly a specific PCR product after large scale amplification. The reaction should be carried out in the usual fashion, except that after optimization of annealing temperature and other conditions in the 5 µl volume, a large scale 50 µl (10X) total reaction volume should be used to generate sufficient PCR product for sequencing.
  http://wheat.pw.usda.gov/~lazo/methods/donis/pcr/p...
  Added: Tue May 14 2002, Hits: 1534, Reviews: 0  Write review   Cached