PEG Purification of PCR Products
This protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cycle-sequencing
Added: Tue May 14 2002, Hits: 1029, Reviews: 0
Purification and Sequencing of PCR Product
This method is used to sequence directly a specific PCR product after large scale amplification. The reaction should be carried out in the usual fashion, except that after optimization of annealing temperature and other conditions in the 5 Ál volume, a large scale 50 Ál (10X) total reaction volume should be used to generate sufficient PCR product for sequencing.
Added: Tue May 14 2002, Hits: 1989, Reviews: 0