Protocols
- DNA Sequencing (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
The sequencing reactions method work perfectly well if you are short of cash to buy sequencing kits. It is based on the Dideoxy sequencing method of Sanger et al., 1977.
http://www.nottingham.ac.uk/~mbzspd/methods/DNA_Se...
Added: Sat Apr 10 2004, Hits: 788, Reviews: 0 Write review Cached - Sequencing (Sequenase) (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Sequencing by the chain-termination method involves the synthesis of a DNA strand by a DNA polymerase using a single stranded template. Synthesis is initiated at the site where an oligonucleotide primer anneals to the template. The synthesis reaction is terminated by the incorporation of a nucleotide analog (ddNTP) that terminates elongation. When proper mixtures of dNTP's and one of the four ddNTP's are used, polymerization will be terminated randomly at each possible site. This protocol covers sequencing gel preparation, sequencing reaction and electrophoresis.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Sat Nov 02 2002, Hits: 494, Reviews: 0 Write review Cached -
Cycle Sequencing Protocol Using 33P-Radiolabeled dITP Terminators
(Department of Carcinogenesis,, The University of Texas M.D. Anderson Cancer Center)
Useful for resolving gel compression resulting from secondary structure in GC-rich regions.
http://sciencepark.mdanderson.org/mbcore/files/Seq...
Added: Tue May 14 2002, Hits: 344, Reviews: 0 Write review -
Cycle Sequencing Protocol Using 33P-Radiolabeled Terminators
(Department of Carcinogenesis,, The University of Texas M.D. Anderson Cancer Center)
Standard protocol for cycle sequencing with radiolabeled dGTP.
http://sciencepark.mdanderson.org/mbcore/files/Seq...
Added: Tue May 14 2002, Hits: 276, Reviews: 0 Write review -
DNA sequencing
(Roe Lab)
In this protocols, the following procedures are introduced:
Bst-catalyzed radiolabeled DNA sequencing
Radiolabeled sequencing gel preparation, loading, and electrophoresis
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
Sequenase[TM] catalyzed sequencing with dye-labeled terminators
Fluorescent-labeled sequencing gel preparation, pre-electrophoresis, sample loading, electrophoresis, data collection, and analysis on the ABI 373A DNA sequencer
Double-stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers
cDNA sequencing based on PCR and random shotgun cloning
http://www.genome.ou.edu/protocol_book/protocol_pa...
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DNA Sequencing Reaction
(Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/sequen...
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Double Stranded DNA Sequencing (Dideoxy)
( )
Double Stranded DNA Sequencing (Dideoxy) ( (Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/mol...
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Double Stranded DNA Sequencing (Dideoxy)
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Sequencing of Double Stranded DNA Using Dideoxy Chain Termination
(Donis Keller Lab)
This method is used to sequence double stranded DNA, such as a plasmid insert or purified PCR product. The dideoxy chain termination (or enzymatic) method of DNA sequencing involves the in vitro synthesis of a DNA strand by a modified bacteriophage T7 DNA polymerase (SequenaseR/USB) using a single stranded DNA template.
http://hg.wustl.edu/hdk_lab_manual/de/de1.html
Added: Tue May 14 2002, Hits: 668, Reviews: 0 Write review Cached