Summary: Analysis of the interaction of proteins with either DNA or RNA sequences by in vivo footprinting involves two steps: (i) the in situ modification of nucleic acids by the footprinting reagent and (ii) the visualization of the footprints. Ligation-mediated PCR (LM-PCR) procedures provide a level of sensitivity and specificity that is suitable for visualization of footprints of single-copy genes or low-abundance mRNAs in higher eukaryotes.
Protocols
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Genomic DNA Isolation for In Vivo Footprinting
(The Morimoto Lab, Northwestern University)
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 29 2004, Hits: 1099, Reviews: 0 Write review -
Ligation-Mediated PCR for Genomic Sequencing & Footprinting
(The Morimoto Lab, Northwestern University)
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 29 2004, Hits: 1078, Reviews: 0 Write review