- Microinjection of Xenopus Oocyte (Gene Delivery Group, University of Birmingham)
This protocol covers: Preparation of the oocytes, Selection of oocytes for microinjection, Sample preparation, Microinjection of the oocytes, Assaying for gene expression.
Added: Mon Sep 09 2002, Hits: 278, Reviews: 0
Oocyte isolation, maturation, enucleation and fractionation
(Klymkowsky Lab )
Added: Mon Jul 22 2002, Hits: 664, Reviews: 0
The aim of the technique is to study the role of these genes by creating a milieu in which the gene is not fully active. This is achieved by reducing the maternal pool of RNA for the gene of interest and studying the effect that depletion has on development. Includes choosing antisense oligonucleotide, modification, host transfer technique, fertilization and development
Added: Fri Aug 02 2002, Hits: 577, Reviews: 0
RNA Extract from Xenopus
(Klymkowsky Lab, Molecular, Cellular & Developmental Biology, University of Colorado, Boulder )
Two methods are offered: TRIzol and phenol:choloform method in great detail. May apply to other samples.
Added: Wed Oct 22 2003, Hits: 651, Reviews: 0