Protocols
-
ABC monoclonal antibody staining of dissected larval and adult CNS
(ITO Kei, JFly)
GFP, X-gal, fluorescent and DAB antibody staining of embryos, larvae, pupae and adult CNSs
http://jfly.nibb.ac.jp/html/manuals/pdf/E_Staining...
Added: Thu Aug 01 2002, Hits: 1017, Reviews: 0 Write review -
Immunohistochemistry on Drosophila Brains
(Vesicle Trafficking, Stanford University)
http://cmgm.stanford.edu/~vesicle/protocols/immuno...
Added: Thu Jul 25 2002, Hits: 1851, Reviews: 0 Write review Cached -
In Situ Hybridisation with DIG-RNA Probes On Embryos
(Ilan Davis)
Protocol for fly including probe preparation
http://homepages.ed.ac.uk/ilan/methods/insitu.txt
Added: Tue May 14 2002, Hits: 2070, Reviews: 0 Write review Cached -
Labelling Drosophila pupae with BrdU
(ITO Kei, JFly)
Staging and BrdU microinjection of pupae
http://jfly.nibb.ac.jp/html/manuals/E_Pupal_Inject...
Added: Thu Aug 01 2002, Hits: 148, Reviews: 0 Write review Cached -
Non-fluorescent protein/RNA double-labeling for fly in situ hybridization
(Henry M. Krause's Lab,University of Toronto)
For embryo in situ hybridization, including method for hybridization
http://www.utoronto.ca/krause/double%20labeling.ht...
Added: Thu Aug 01 2002, Hits: 650, Reviews: 0 Write review Cached -
Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
(JoVE)
This protocol presents a histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. These preparations allow for the tissue to be stained with classical histological stains and labeled with protein detecting dyes, and specifically cryosections are ideal for immunohistochemical detection of proteins in intact muscles.
http://www.jove.com/details.php?id=2438
Added: Sat Apr 23 2011, Hits: 330, Reviews: 0 Write review -
Single and Double FISH protocols for Drosophila
(Krause Lab)
http://www.utoronto.ca/krause/FISH.html
Added: Tue May 14 2002, Hits: 397, Reviews: 0 Write review Cached -
Staining using lipophilic fluorescent dyes
(ITO Kei, JFly)
DiI microinjection and photoconversion
http://jfly.nibb.ac.jp/html/manuals/pdf/E_DiI_Stai...
Added: Thu Aug 01 2002, Hits: 720, Reviews: 0 Write review -
X-gal Staining of Wholemount Drosophila Embryos
(UEMURA Tadashi, JFly)
X-gal staining of embryos and imaginal discs, antibody staining of embryos.
http://jfly.nibb.ac.jp/html/manuals/pdf/E_Staining...
Added: Thu Aug 01 2002, Hits: 932, Reviews: 0 Write review