Top : Model Organisms : Drosophila : Drosophila Culture & Handling
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Protocols
  • Drosophila Media Recipes (The Bloomington Drosophila Stock Center, Indiana University)
    • Standard medium 
    • Hard agar medium 
    • Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium 
    • Cornmeal-molasses-yeast medium 
    • Cornmeal-sucrose-yeast medium 
    • Sucrose-yeast medium 
    • Cornmeal-dextrose-yeast medium 
    • Semi-defined medium

    http://flystocks.bio.indiana.edu/media-recipes.htm
    Added: Thu Aug 01 2002, Hits: 201, Reviews: 0  Write review   Cached
  • Basic Methods of Culturing Drosophila (The Bloomington Drosophila Stock Center, Indiana University)
    Detailed method for culturing fly.
    http://flystocks.bio.indiana.edu/culturing.htm
    Added: Thu Aug 01 2002, Hits: 293, Reviews: 0  Write review   Cached
  • Culture of Drosophila Cells (Drosophila RNAi Screening Center at Harvard Medical School)
    Covering culture medium, growth conditions, maintainance, freezing and thawing...
    http://flyrnai.org/RNAi_FAQ_dros_cc.html
    Added: Wed May 12 2004, Hits: 524, Reviews: 0  Write review   Cached
  • Current Bloomington Recipe for Drosophila Medium (The Bloomington Drosophila Stock Center, Indiana University )
    Includes the recipe, cooking instructions and ingredients notes.
    http://flystocks.bio.indiana.edu/bloom-food.htm
    Added: Thu Aug 01 2002, Hits: 100, Reviews: 0  Write review   Cached
  • Cuticle preparations (Henry M. Krause's Lab,University of Toronto)
    http://www.utoronto.ca/krause/cuticles.html
    Added: Thu Aug 01 2002, Hits: 199, Reviews: 0  Write review   Cached
  • Dissection of Drosophila CNSs PDF file (ITO Kei, JFly)
    Preparation of dissection tools, How to dissect brains of pupae and adults
    http://jfly.nibb.ac.jp/html/manuals/pdf/E_CNS_Diss...
    Added: Thu Aug 01 2002, Hits: 420, Reviews: 0  Write review  
  • Growing imaginal disc cells (Nusse Lab, Stanford University)
    general maintenance instructions for imaginal disc cell lines
    http://www.stanford.edu/~rnusse/ownpage/protdiscce...
    Added: Thu Aug 01 2002, Hits: 73, Reviews: 0  Write review   Cached
  • Hard Agar Medium for Egg Collection Plates (The Bloomington Drosophila Stock Center, Indiana University )
    This is a firm medium that keeps well and makes a good base for egg collecting
    http://flystocks.bio.indiana.edu/hard-agar.htm
    Added: Thu Aug 01 2002, Hits: 78, Reviews: 0  Write review   Cached
  • Methods for the preparation of Fly Extract (School of Biology, University of St. Andrews)
    For primary and routine culture
    http://www-sbms.st-and.ac.uk/sites/flycell/flyextr...
    Added: Thu Aug 01 2002, Hits: 109, Reviews: 0  Write review   Cached
  • production of wingless protein (Nusse Lab, Stanford University )
    Production of Conditioned Medium containing Wingless protein from S2 cells
    http://www.stanford.edu/~rnusse/ownpage/production...
    Added: Thu Aug 01 2002, Hits: 59, Reviews: 0  Write review   Cached
  • Routine methods for growing the cell lines (School of Biology, University of St. Andrews)
    Medium/Insulin/Calcium- and magnesium- free saline/Passaging cell lines/Cell seeding numbers
    http://www-sbms.st-and.ac.uk/sites/flycell/growing...
    Added: Thu Aug 01 2002, Hits: 154, Reviews: 0  Write review   Cached
  • Single-Fly DNA Preparations for PCR (Lazo Lab)
    A simple method for the rapid and reproducible isolation of DNA from single flies for amplification by PCR and direct sequencing by asymmetric PCR.
    http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafly...
    Added: Tue May 14 2002, Hits: 360, Reviews: 0  Write review   Cached