Protocols
- Simplified Arabidopsis Transformation Protocol (The Arabidopsis Information Resource)
With this method you should be able to achieve transformation rates above 1% (one transformant for every 100 seed harvested from Agrobacterium-treated plants).
http://www.arabidopsis.org/info/transformation.htm...
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Agrobacterium competent cells
(The Arabidopsis Information Resource)
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
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Agrobacterium electroporation
(The Arabidopsis Information Resource)
Safety precautions/DNA preparations/Preparing the electroporator/Electroporating/Re-using cuvettes.
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
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Agrobacterium miniprep (phenol free)
(The Arabidopsis Information Resource)
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
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Arabidopsis transformation (infiltration)
(The Arabidopsis Information Resource)
This protocol is modified from Bechtold, Ellis and Pelletier (1993). "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants".
http://www.arabidopsis.org/info/Protocols_Mundy2.h...
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Competent Agro Preparation for Electroporation
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
http://www.bch.msu.edu/pamgreen/agro.htm
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Transformation of Arabidopsis Thaliana by Vacuum Infiltration
(Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
This protocol gives very good results, with at least 95% of all infiltrated plants giving rise to transformants, and a transformant rate of 1-4% of seed.
http://www.bch.msu.edu/pamgreen/vac.htm
Added: Sat Oct 25 2003, Hits: 80, Reviews: 0 Write review Cached