Sponsored Links:

Protocols

• Simplified Arabidopsis Transformation Protocol (The Arabidopsis Information Resource)
  With this method you should be able to achieve transformation rates above 1% (one transformant for every 100 seed harvested from Agrobacterium-treated plants).
  http://www.arabidopsis.org/info/transformation.htm...
  Added: Tue Jul 30 2002, Hits: 342, Reviews: 0  Write review   Cached
• Agrobacterium competent cells (The Arabidopsis Information Resource)
  http://www.arabidopsis.org/info/Protocols_Mundy2.h...
  Added: Tue Jul 30 2002, Hits: 228, Reviews: 0  Write review   Cached
• Agrobacterium electroporation (The Arabidopsis Information Resource)
  Safety precautions/DNA preparations/Preparing the electroporator/Electroporating/Re-using cuvettes.
  http://www.arabidopsis.org/info/Protocols_Mundy2.h...
  Added: Tue Jul 30 2002, Hits: 276, Reviews: 0  Write review   Cached
• Agrobacterium miniprep (phenol free) (The Arabidopsis Information Resource)
  http://www.arabidopsis.org/info/Protocols_Mundy2.h...
  Added: Tue Jul 30 2002, Hits: 217, Reviews: 0  Write review   Cached
• Arabidopsis transformation (infiltration) (The Arabidopsis Information Resource)
  This protocol is modified from Bechtold, Ellis and Pelletier (1993). "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants".
  http://www.arabidopsis.org/info/Protocols_Mundy2.h...
  Added: Tue Jul 30 2002, Hits: 188, Reviews: 0  Write review   Cached
• Competent Agro Preparation for Electroporation (Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
  http://www.bch.msu.edu/pamgreen/agro.htm
  Added: Sat Oct 25 2003, Hits: 52, Reviews: 0  Write review   Cached
• Transformation of Arabidopsis Thaliana by Vacuum Infiltration (Green Lab, U.S. Department of Energy-Plant Research Laboratories at Michigan State University)
  This protocol gives very good results, with at least 95% of all infiltrated plants giving rise to transformants, and a transformant rate of 1-4% of seed.
  http://www.bch.msu.edu/pamgreen/vac.htm
  Added: Sat Oct 25 2003, Hits: 80, Reviews: 0  Write review   Cached