Protocols
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Cell Suspension Culture of Arabidopsis
(BioProtocol)
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Pollen Development in Anthers of Arabidopsis
(BioProtocol)
Using this procedure, it is possible to follow the development of pollen mother cells through to mature pollen. Sterile males were made using irradiation mutagenesis of Landsberg erecta seed. Buds harvested from the primary inflorescence just before bolting seem to give more consistent results (reasonable chromosome pictures) than buds that develop later. Buds are harvested and fixed from populations that are known to segregate sterile males. Plants are allowed to flower and bolt a second time. At this stage plants are scored for sterility or fertility.
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Added: Sun Jul 21 2002, Hits: 94, Reviews: 0 Write review Cached