Protocols
- Detection of Viruses in Infected Plant Extracts using Immunocapture-PCR (Dr. Sephra Rampersad, The University of the West Indies, Department of Life Sciences)
Diagnostic PCR should fulfill several qualitative characteristics, but the most important among them are specificity, sensitivity, efficiency and reproducibility of detection of viruses. Immunocapture-PCR (IC-PCR) may be used in detecting viruses using clarified plant extracts with degenerate primers, without prior isolation of total nucleic acids. It is especially useful for detecting viruses that exist in low or variable titers en planta and in plant species which contain various forms of PCR amplification inhibitors (polysaccharides, tannins, polyphenolics etc.).
Added: Thu Feb 03 2005, Reviews: 0 Write review -
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells (Journal of Visualized Experiments )
This video protocol demonstrates an efficient way to prepare replication-defective retroviral stocks encoding a human oncogene, and subsequently used for induction of myeloproliferative disease in the mouse model.
http://www.jove.com/index/Details.stp?ID=550
Added: Mon Feb 25 2008, Hits: 406, Reviews: 0 Write review Cached -
Creation and Use of Infectious Virus Vector
(Stewart Lab, Washington University at St. Louis)
http://www.stewartlab.wustl.edu/Making_Virus.htm
Added: Thu May 27 2004, Hits: 2172, Reviews: 0 Write review Cached -
Generation of recombinant baculoviruses by co-transfection
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
Added: Wed Aug 14 2002, Hits: 2018, Reviews: 0 Write review -
Notes on Transgenic Resistance to Plant Viruses
(Ayeepettai, Vegakollai Post, Panruti T.K, 302 Cuddalore, India)
General notes on transgenic resistance to plant viruses with few references given in PDF format.
http://www.protocol-online.org/files/protocol_3485...
Added: Sat Jan 29 2005, Hits: 542, Reviews: 0 Write review -
Production of Adenoviral Vectors
(A. Untergasser)
A protocol describing how to produce adenoviral vectors starting from plasmid DNA.
http://www.untergasser.de/lab/protocols/adeno_vect...
Added: Sun Mar 30 2008, Hits: 82, Reviews: 0 Write review -
Retroviral infection of primary human keratinocytes (PHKs)
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/cellcult/infectn.html
Added: Fri Sep 06 2002, Hits: 1656, Reviews: 0 Write review Cached -
Titration of Adenoviral Vectors
(A. Untergasser)
A protocol describing how to measure the concentration of infectious units in a freshly produced virus stock.
http://www.untergasser.de/lab/protocols/adeno_vect...
Added: Sun Mar 30 2008, Hits: 129, Reviews: 0 Write review -
Titration of Retrovirus
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/virus/titratn.html
Added: Fri Sep 06 2002, Hits: 3852, Reviews: 0 Write review Cached