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Protocols
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Creation and Use of Infectious Virus Vector
(Stewart Lab, Washington University at St. Louis)
http://www.stewartlab.net/Protocols/Making_Virus_1...
Added: Thu May 27 2004, Hits: 1211, Reviews: 0 Write review Cached -
Generation of recombinant baculoviruses by co-transfection
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
Added: Wed Aug 14 2002, Hits: 1467, Reviews: 0 Write review -
Retroviral infection of primary human keratinocytes (PHKs)
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/cellcult/infectn.html
Added: Fri Sep 06 2002, Hits: 913, Reviews: 0 Write review Cached