Protocols

• Agglutination Assays (Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
  Two methods are described: bacteria agglutination and passive agglutination.
  http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
  Added: Sat Aug 31 2002, Hits: 1716, Reviews: 0  Write review   Cached
• Antimicrobial Susceptibility Testing by Disc Diffusion Method. (Protocol Online)  update
  Standard protocol for antimicrobial sensitivity testing by disc diffusion method.
  Added: Fri Mar 30 2007, Reviews: 0  Write review  
• Chemical Gradient Motility Agar (CGMA): A System, demonstrating chemoreceptor mediated chemotaxis. (Abhishek D. Garg, Dept. of Biotech. & Biochem., G. H. Raisoni National College, Pt. Ravishankar Shukla University, Raipur.)
  Chemical gradient motility agar (CGMA) is a bacterial assay which is utilizable for efficient demonstration of positive as well as negative bacterial chemotaxis in a common Petri dish environment. This system has been created originally at our lab to supplement proper investigation of bacterial chemotaxis.
  http://www.protocol-online.org/files/protocol_4174...
  Added: Wed Nov 22 2006, Hits: 356, Reviews: 0  Write review  
• Gram Stain Protocol - Hucker's Modification (David B. Fankhauser, University of Cincinnati Clermont College, Batavia)
  Both Gram-positive (Gm+) and Gram-negative (Gm) organisms form a complex of crystal violet and iodine within the bacterial cell during the Gram-staining procedure. Gm+ organisms are thought to resist decolorization by alcohol or acetone because cell wall permeability is markedly decreased when it is dehydrated by these solvents. Thus, the dye complex is entrapped within the cell, resist being washed out by the solvents, and Gm+ bacteria remain purple following this differential stain.
  http://biology.clc.uc.edu/fankhauser/Labs/Microbio...
  Added: Wed Aug 31 2005, Hits: 1333, Reviews: 0  Write review   Cached
• Gram Staining (+-) (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
  Added: Tue May 14 2002, Hits: 1950, Reviews: 0  Write review   Cached
• Gram-Staining Procedure (MEDIC, U of Texas)
  Very nice and detailed method description for Gram staining
  http://medic.med.uth.tmc.edu/path/grampro.htm
  Added: Tue May 14 2002, Hits: 2052, Reviews: 0  Write review   Cached
• Preparation of E Coli Protein for SDS-PAGE (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
  Simplified method for analyzing E coli protein
  http://www.fhcrc.org/labs/hahn/methods/biochem_met...
  Added: Tue May 14 2002, Hits: 1310, Reviews: 0  Write review   Cached
• Smear Preparation (MEDIC, U of Texas)
  The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium.
  http://medic.med.uth.tmc.edu/path/smear.htm
  Added: Tue May 14 2002, Hits: 1278, Reviews: 0  Write review   Cached