- Indirect ELISA (Abcam)
For accurate quantitative results, always compare signal of unknown samples against those of a standard curve. Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy.
Added: Sat Mar 14 2009, Hits: 8979, Reviews: 0
(Kitto Lab, The University of Texas at Austin)
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones.
Added: Tue Oct 08 2002, Hits: 6295, Reviews: 0
Added: Sat Mar 14 2009, Hits: 4267, Reviews: 0