Summary: Tissue Microarrays (TMAs) or tissue array or tissue chips, originally described by in 1987 by Wan, Fortuna and Furmanski and popularized by Juha Kononen, is a powerful new technology for high throughput analysis of protein expression in a large number of tissue samples. Hundreds or thousands of tissue cores are arranged on a single slide, and then analyzed by a single immuno-staining or in situ hybridization reaction.
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Protocols
- The Principles, Uses and Construction of Tissue Microarrays (TMA Laboratory Johns Hopkins Pathology)
Detailed description of TMA uses, validation, design, construction, data handling, and image handling
http://tmalab.jhmi.edu/DeMarzo_Fedor_TMA.html
Added: Sun Apr 05 2009, Hits: 3057, Reviews: 0 Write review - Tissue Microarray Protocol
(Yale Cancer Center Tissue Microarray Facility)
Offers detailed protocol and guide on this new technique.
http://www.yalepath.org/dept/research/YCCTMA/YTMA_...
Added: Thu Mar 25 2004, Hits: 4157, Reviews: 0 Write review -
Creating an Interactive Database for Image Viewing & Data Input
(Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University )
The major problem of using tissue microarray is how to simultaneously examine each core at the microscope, know its identity and input the score in a database next to the correct identifier. Here offers you simple methods of using Excel to achieve these goals.
http://icg.cpmc.columbia.edu/cattoretti/Protocol/i...
Added: Thu Mar 25 2004, Hits: 528, Reviews: 0 Write review Cached -
Protocol for Processing Cultured Cells for Tissue Microarrays
(Yale University Tissue Microarray Facility)
This protocol was developed to provide a standard size cell disk for coring
http://www.tissuearray.org/CellPelletProtocolforTM...
Added: Sun Apr 05 2009, Hits: 2100, Reviews: 0 Write review -
Tissue Micro-array Construction and Storage
(AIDS and Cancer Specimen Resource (ACSR), UCSF)
http://acsr.ucsf.edu/SOP/Tissue_Microarray_SOP_rev...
Added: Sun Apr 05 2009, Hits: 2059, Reviews: 0 Write review