Protocols
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C. Elegans Gene Knockout
(Vancouver Gene Knockout Laboratory, at the University of British Columbia)
The procedure is to mutagenize a large population of worms with trimethylpsoralen and UV irradiation, set up 1152 subpopulations, screen DNA made from this library for deletions in specific genes by nested PCR, and then to recover single worms carrying the deletions through a sib-selection process.
http://ko.cigenomics.bc.ca/protocols.html
Added: Tue May 14 2002, Hits: 445, Reviews: 0 Write review Cached -
C. Elegans Gene Knockout
(Michael Koelle)
The gene knockout strategy involves using a random mutagen (trimethylpsoralen) to mutagenize a very large number of worms. The worms are divided into many small subcultures and allowed to have progeny. A portion of each subculture is stored alive in the freezer, and genomic DNA is made...
http://info.med.yale.edu/mbb/koelle/protocols/prot...
Added: Tue May 14 2002, Hits: 596, Reviews: 0 Write review -
Electroporation of ES Cells and Isolation of H/R Clones #2
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Gene Knockout In Murine Embryonic Stem (ES) Cells #1
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Gene Targeting Outline
(University of Michigan Transgenic Animal Model Core)
This is a brief outline of the steps necessary to produce mice with a mutation targeted to a specific gene. These animals are referred to as "knock-out" mice or "gene targeted" mice
http://www.med.umich.edu/tamc/esoutline.html
Added: Tue May 14 2002, Hits: 1343, Reviews: 0 Write review Cached