Top : Genetics & Genomics : Microarray : Nucleic Acid Labeling
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Protocols
  • Amino-Allyl cDNA Labeling (Fero Lab, Fred Hutchinson Cancer Research Center)
    http://labs.fhcrc.org/fero/Protocols/AAcDNAlabelin...
    Added: Sat Jan 31 2009, Hits: 306, Reviews: 0  Write review   Cached
  • Amplification and Labeling of DNA PDF file (DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
    Protocol for amplifying and labeling DNA from ChIP or elsewhere
    http://www.microarrays.org/pdfs/Round_A_B_C.pdf
    Added: Tue Sep 24 2002, Hits: 651, Reviews: 0  Write review  
  • E.coli Genomic DNA Labeling (E. coli Genome Project at UW-Madison)
    Label fragmented E.coli genomic DNA with CyDye-dUTP directly using Klew Fragment.
    http://www.genome.wisc.edu/resources/protocols/gen...
    Added: Tue Sep 24 2002, Hits: 325, Reviews: 0  Write review   Cached
  • E.coli Total RNA Labeling Protocol for Spotted Microarray (UW E. coli Genome Project)
    Label E.coli Total RNA with CyDye-dUTP directly using Invitrogen Superscript II reverse transcriptase. RNA preparation/labeling/purification
    http://www.genome.wisc.edu/resources/protocols/rna...
    Added: Mon Aug 05 2002, Hits: 348, Reviews: 0  Write review   Cached
  • Labeling Genomic DNA PDF file (DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
    Protocols for labeling genomic DNA via direct incorporation or coupling for comparative genomic hybridizations.
    http://www.microarrays.org/pdfs/GenomicDNALabel_B....
    Added: Tue Sep 24 2002, Hits: 520, Reviews: 0  Write review  
  • Preparation of Fluorescent DNA Probe from Genomic DNA (Pat Brown Lab, Department of Biochemistry and the Howard Hughes Medical Institute)
    Developed for microarray-based comparative genomic hybridization. Genomic DNA can be labeled with a simple random-priming protocol based on Gibco/BRL's Bioprime DNA Labeling kit, though nick translation protocols work too.
    http://cmgm.stanford.edu/pbrown/protocols/4_genomi...
    Added: Tue May 14 2002, Hits: 422, Reviews: 0  Write review   Cached
  • Preparation of Fluorescent DNA Probe from Yeast mRNA  (Brown Lab)
    http://cmgm.stanford.edu/pbrown/protocols/4_yeast_...
    Added: Tue May 14 2002, Hits: 264, Reviews: 0  Write review   Cached
  • Preparation of Fluorescent Probe from E. coli RNA (Pat Brown Lab, Department of Biochemistry and the Howard Hughes Medical Institute)
    Total Bacterial RNA Labeling with Random Hexamers
    http://cmgm.stanford.edu/pbrown/protocols/4_Ecoli_...
    Added: Tue May 14 2002, Hits: 275, Reviews: 0  Write review   Cached
  • Preparation of Fluorescent Probe from Human RNA (Pat Brown Lab, Department of Biochemistry and the Howard Hughes Medical Institute)
    Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
    http://cmgm.stanford.edu/pbrown/protocols/4_human_...
    Added: Tue May 14 2002, Hits: 299, Reviews: 0  Write review   Cached
  • Reverse Transcription and aa-UTP Labeling of RNA (Pat Brown Lab, Department of Biochemistry and the Howard Hughes Medical Institute)
    Detailed protocol of labeling RNA using amino-allyl Reverse Transcription
    http://cmgm.stanford.edu/pbrown/protocols/aadUTPCo...
    Added: Tue Aug 06 2002, Hits: 343, Reviews: 0  Write review   Cached
  • Reverse Transcription and aa-UTP Labeling of RNA PDF file (DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
    Protocol for making cDNA with aa-dUTP incorporation and labeling cDNA via chemical coupling of fluorescent dyes.
    http://www.microarrays.org/pdfs/amino-allyl-protoc...
    Added: Tue Sep 24 2002, Hits: 391, Reviews: 0  Write review  
  • Reverse Transcription and Labeling of Human RNA PDF file (DeRisi Lab, Dept. of Biochemistry & Biophysics, Univ. of California at San Francisco)
    Protocol for direct incorporation of fluorescent nucleotides into cDNA reverse transcribed from human mRNA
    http://www.microarrays.org/pdfs/HumanRNALabel.pdf
    Added: Tue Sep 24 2002, Hits: 399, Reviews: 0  Write review  
  • Revised amino-allyl labeling (Pat Brown Lab, Department of Biochemistry and the Howard Hughes Medical Institute)
    Reverse Transcription and Amino-allyl Coupling of RNA
    http://cmgm.stanford.edu/pbrown/protocols/amino-al...
    Added: Tue Aug 06 2002, Hits: 297, Reviews: 0  Write review   Cached