Protocols

• 3% Trevigel Protocol for 3 bp resolution of SSR PCR products (Dendrome Project, UC Davis)
  http://dendrome.ucdavis.edu/colleague/protocol_det...
  Added: Tue May 14 2002, Hits: 646, Reviews: 0  Write review   Cached
• A Rapid CTAB DNA Isolation Technique Useful for RAPD Fingerprinting and Other PCR Applications (Dendrome Project, UC Davis)
  http://dendrome.ucdavis.edu/colleague/protocol_det...
  Added: Tue May 14 2002, Hits: 1763, Reviews: 0  Write review   Cached
• Amplified Fragment Length Polymorphisms (AFLPs) (Oregon State U)
  http://www.css.orst.edu/Barley/BREEDING/aflps.htm
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• Chemiluminescent AFLP protocol (CIMMYT Applied Molecular Genetics Laboratory)
  After amplification and electrophoresis on sequencing gels, the amplification products are transfered to a nylon membrane, and the anti-Dig / alkaline phosphatase and CSPD system is used to detect on an X-ray film the amplification products.
  http://www.cimmyt.org/english/docs/manual/protocol...
  Added: Fri Aug 02 2002, Hits: 608, Reviews: 0  Write review  
• Denaturing Gradient Gel Electrophoresis (DGGE) (Donis-Keller lab)
  Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially the fragments move according to molecular weight, but as they progress into higher denaturing conditions, each (depending on its sequence composition) reaches a point where the DNA begins to melt. The partial melting severely retards the progress of the molecule in the gel, and a mobility shift is observed. It is the mobility shift which can differ for slightly different sequences (depending on the sequence, as little as a single bp change can cause a mobility shift). Alleles are detected by differences in mobility.
  http://humgen.wustl.edu/hdk_lab_manual/dgge/dgge1....
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• RAPD Reaction and Cycling Conditions (Kim Marshall)
  http://dendrome.ucdavis.edu/colleague/protocol_det...
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• SSR Gel and Silver Staining Protocol (Dendrome Project, UC Davis)
  http://dendrome.ucdavis.edu/colleague/protocol_det...
  Added: Tue May 14 2002, Hits: 1244, Reviews: 0  Write review   Cached