Summary: RNA-FISH is a FISH technique modified for studying RNA processing, and quantitation, and has also been used to determine allele-specific expression of genes. The RNA FISH technique requires the generation of a labeled probe, hybridization of the probe to a fixed sample, and subsequently, detection of the labeled probe using microscopy.
- RNA FISH on cultured cells in interphase (Epigenome Network of Excellence)
Fluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly versatile and has been adapted to carry out genome-wide screenings, microarray quantifications, cancer cytogenetics analysis, and RNA expression and localization studies. The study of intracellular RNA localization using RNA FISH provides insights into the in situ physical characteristics of transcription and intracellular RNA transport in individual cells. In our lab, we use RNA FISH to detect the localization of Xist RNA, a nuclear non-coding transcript that coats the entire chromosome from which it is transcribed.
Added: Wed Feb 04 2009, Hits: 1021, Reviews: 0
- Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FI (Singer Lab, Yeshiva University)
Added: Sat Feb 21 2009, Hits: 1261, Reviews: 0
RNA Fish Protocol
(The Bird Lab, The University of Edinburgh)
Added: Wed Feb 04 2009, Hits: 2610, Reviews: 0
(Reijo Pera Lab, Stanford University)
Added: Wed Feb 04 2009, Hits: 1586, Reviews: 0
Sequential RNA and DNA fluorescence in situ hybridization (FISH)
(Epigenome Network of Excellence)
The most efficient way to map gene loci and their activity is sequential RNA and DNA FISH. This is an important technique to uncover how gene positioning is linked to activity.
Added: Wed May 27 2009, Hits: 789, Reviews: 0