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Protocols
  • Comparative Genomic Hybridization (Molecular Profiling Initiative, NCI)
    Comparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number changes throughout the genome in a single hybridization. CGH is based on co-hybridization of differentially labeled tumor and normal DNA to human metaphase chromosomes.
    http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
    Added: Tue May 14 2002, Hits: 294, Reviews: 0  Write review   Cached
  • Comparative Genomic Hybridization (CGH) (Institute of Pathology,Humboldt-University of Berlin)
    CGH is a molecular Cytogenetic method of screening a tumor for genetic changes. The alterations are classified as DNA gains and losses and reveal a characteristic pattern that includes mutations at chromosomal and subchromosomal levels. This protocol gives detailed procedures on CGH as follows:Metaphase chromosome preparation, DNA preparation by cryostom tissue dissection, DNA labeling by nick translation
    Hybridization, DNA detection, Image capture, Image analysis, DAPI chromosome identification
    http://amba.charite.de/cgh/protocol/02/prot02.html
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  • Comparative Genomic Hybridization (CGH) (Institute of Pathology,Humboldt-University of Berlin)
    Detailed protocol for CGH, including slide and probe preparation, detection and staining
    http://allserv.rug.ac.be/~fspelema/neubla/protocol...
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  • Direct CGH (Waldman Lab)
    http://cc.ucsf.edu/people/waldman/Protocols/direct...
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  • Indirect CGH (Waldman Lab)
    http://cc.ucsf.edu/people/waldman/Protocols/indire...
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  • Nick Translation for CGH PDF file (Thomas Ried's Lab, NCI)
    Label with biotinylated dUTP
    http://www.riedlab.nci.nih.gov/publications/2403.0...
    Added: Sat Oct 19 2002, Hits: 844, Reviews: 0  Write review  
  • Nick Translation of DNA for CGH (Waldman Lab)
    http://cc.ucsf.edu/people/waldman/Protocols/nicktr...
    Added: Tue May 14 2002, Hits: 888, Reviews: 0  Write review   Cached