Protocols
- Use of Micropipets (Department of Biology, Bates College)
Very basic guide on use of pipets.
http://abacus.bates.edu/~ganderso/biology/resource...
Added: Sat Nov 01 2003, Hits: 1403, Reviews: 0 Write review Cached -
Disposal of Buffers and Chemicals
(Julie B. Wolf, University of Maryland, Baltimore County)
http://userpages.umbc.edu/~jwolf/method2c.htm
Added: Tue May 14 2002, Hits: 1046, Reviews: 0 Write review Cached -
General Laboratory Procedures, Equipment Use, and Safety Considerations
(Julie B. Wolf, University of Maryland, Baltimore County)
http://userpages.umbc.edu/~jwolf/method2d.htm
Added: Tue May 14 2002, Hits: 962, Reviews: 0 Write review Cached - How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
Added: Tue May 14 2002, Reviews: 0 Write review -
How to start in a new Lab - The Ultimate Newbie Guide!
(A. Untergasser)
This is a guide every student making his first steps in a lab should read. It mentions the most common mistakes that starters do because they don't know how to deal with the strange scientists around them. Should be fun to read.
http://www.untergasser.de/lab/howtos/start_up_new_...
Added: Sun Mar 30 2008, Hits: 212, Reviews: 0 Write review -
Phenol Equilibration
(Marcantonio Lab)
http://home.earthlink.net/~erikyoung/protcls.html#...
Added: Tue May 14 2002, Hits: 556, Reviews: 0 Write review Cached -
Preparation and Use of a Dry Ice / Ethanol Bath
(BioProtocol)
Dry Ice / Ethanol baths can be used to freeze tissue sections for histology or to rapidly cool Ethanol solutions to induce the precipitation of RNA, DNA, or proteins.
http://www.bioprotocol.com/protocolstools/protocol...
Added: Fri Mar 07 2008, Hits: 114, Reviews: 0 Write review -
Preparation of Solutions
(Julie B. Wolf, University of Maryland, Baltimore County)
http://userpages.umbc.edu/~jwolf/method2b.htm
Added: Tue May 14 2002, Hits: 2601, Reviews: 0 Write review Cached -
Preparation of Sonicated Human DNA
(Donis Keller Lab)
To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern and in situ hybridizations. This method can also be used for sonicating salmon sperm DNA.
http://humgen.wustl.edu/hdk_lab_manual/southern/so...
Added: Tue May 14 2002, Hits: 453, Reviews: 0 Write review Cached -
Reusing Planting Plates
(by Greg Stuart)
A protocol for cleaning and reusing the large 25 x 25 cm plates
http://eden.ceh.uvic.ca/cleaning.htm
Added: Tue May 14 2002, Hits: 133, Reviews: 0 Write review Cached