Protocols
- Use of Micropipets (Department of Biology, Bates College)
Very basic guide on use of pipets.
http://abacus.bates.edu/~ganderso/biology/resource...
Added: Sat Nov 01 2003, Hits: 596, Reviews: 0 Write review Cached -
Disposal of Buffers and Chemicals
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/method2.htm#Di...
Added: Tue May 14 2002, Hits: 707, Reviews: 0 Write review Cached -
Equipment
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/method2.htm#Eq...
Added: Tue May 14 2002, Hits: 629, Reviews: 0 Write review Cached - How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
Added: Tue May 14 2002, Reviews: 0 Write review -
Phenol Equilibration
(Marcantonio Lab)
http://home.earthlink.net/~erikyoung/protcls.html#...
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Preparation of Solutions
(Julie B. Wolf, University of Maryland, Baltimore County)
http://www.research.umbc.edu/~jwolf/method2.htm#Pr...
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Preparation of Sonicated Human DNA
(Donis Keller Lab)
To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern and in situ hybridizations. This method can also be used for sonicating salmon sperm DNA.
http://hg.wustl.edu/hdk_lab_manual/southern/southe...
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Reusing Planting Plates
(by Greg Stuart)
A protocol for cleaning and reusing the large 25 x 25 cm plates
http://eden.ceh.uvic.ca/cleaning.htm
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Serial Dilution
(John Roth, Division of Biological Sciences,University of California at Davis)
Making serial dilution of a chemical solution, or a suspension of bacteria or bacteriophage
http://rothlab.ucdavis.edu/protocols/M04.html
Added: Sun Jun 06 2004, Hits: 729, Reviews: 0 Write review Cached -
Silane and Poly-L-Lysine Coating of Microscope Slides
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
http://www.nottingham.ac.uk/~mbzspd/methods/Silane...
Added: Sat Apr 10 2004, Hits: 59, Reviews: 0 Write review Cached -
Siliconizing Glassware
(Gerard Lazo)
http://wheat.pw.usda.gov/~lazo/methods/lazo/silico...
Added: Tue May 14 2002, Hits: 314, Reviews: 1 Read reviews Write review Cached -
UV Shadowing
(Ambion)
UV shadowing is a technique for visualizing nucleic acids, e.g. separated in a gel, using short-wave UV light (254 nm) and a fluor-coated TLC plate. This method is for identifying nonisotopic probes or unlabeled RNA (e.g. for microinjection) during gel purification since the other alternative of staining with EtBr or acridine orange requires subsequent extraction of the dye. However, this method can be used to visualize any nucleic acid present in sufficient amount (> 0.3 µg).
http://www.ambion.com/techlib/tips/uv-shadowing.ht...
Added: Tue May 14 2002, Hits: 542, Reviews: 0 Write review Cached