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Protocols
- The Restriction Enzyme Database (REBASE) (REBASE)
contains a complete listing of all known restriction endonucleases, including: recognition sequences, methylation sensitivity, commercial availability and references. This work has been funded for many years by grants from the NSF and NIH to Dr. Richard J. Roberts.
http://rebase.neb.com/rebase/rebase.html
Added: Sun Jul 21 2002, Hits: 6495, Reviews: 0
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Alphabetic List of Commercial Restriction Endonucleases and Their Isoschizomers
(Fermentas)
http://www.fermentas.com/techinfo/RE/restrcommercr...
Added: Tue May 14 2002, Hits: 2353, Reviews: 0
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Commercial Restriction Endonucleases Sorted by Generated DNA Ends
(Fermentas)
Enzymes Generating 5'-protruding Ends
Enzymes Generating 3'-protruding Ends
Enzymes Generating Blunt Ends
Enzymes Cleaving DNA on Both Sides of Their Recognition Sequence
http://www.fermentas.com/techinfo/RE/restrcommre_d...
Added: Tue May 14 2002, Hits: 2077, Reviews: 0
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Factors that Influence Restriction Enzyme Activity
(Dr. Bowen et al, Colorado State University)
At times, the DNA does not appear to cut at all and sometimes it cuts only partially. This is a good summary of factors that may influence restriction activities.
http://arbl.cvmbs.colostate.edu/hbooks/genetics/bi...
Added: Wed May 11 2005, Hits: 3286, Reviews: 0
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Star Activity
(NEB)
Conditions that contribute to Star Activity and how to inhibit star activity.
http://www.biochem.northwestern.edu/holmgren/Gloss...
Added: Wed May 11 2005, Hits: 1693, Reviews: 0
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Star Activity of Restriction Enzymes
(Takara)
There are some restriction enzymes which cleave partially different base sequence than recognition sequence as a result of reduced specificity when used in a large excess to substrate DNA. This phenomenon is called Star Activity. The page lists enzymes for which Star Activity is noticed, the conditions to accelerate its appearance, and the base sequences cleaved by Star Activity for which such reports are available.
http://bio.takara.co.jp/BIO_EN/catalog_d.asp?C_ID=...
Added: Wed May 11 2005, Hits: 1214, Reviews: 0
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