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  • Lab Protocols in Developmental Biology (Experiments in Developmental Biology)
    A collection of protocols on Sea Urchin, Zebrafish, amphibians (Xenopus & axolotl), chick,and mouse
    http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/D...
    Added: Wed Mar 04 2009, Hits: 509, Reviews: 0  Write review   Cached
  • Cell number counting in preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
    A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number
    http://www.animal.ufl.edu/hansen/protocols/embryoc...
    Added: Tue May 14 2002, Hits: 723, Reviews: 0  Write review   Cached
  • In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
    This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos
    http://www.animal.ufl.edu/hansen/IVF/default.htm
    Added: Tue May 14 2002, Hits: 477, Reviews: 0  Write review   Cached
  • Lineage Analysis Using Retrovirus Vectors (Cepko Lab, Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute,)
    Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.
    http://axon.med.harvard.edu/~cepko/lineage/
    Added: Tue May 14 2002, Hits: 439, Reviews: 0  Write review   Cached
  • Mini-Chamber for regulating gaseous environment during culture (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
    A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device
    http://www.animal.ufl.edu/hansen/protocols/minigas...
    Added: Tue May 14 2002, Hits: 364, Reviews: 0  Write review   Cached
  • Sexing Bovine Preimplantation Embryos by PCR PDF file (PJ Hansen Laboratory)
    a simple PCR based protocol for distinquishing between male and female embryos
    http://www.animal.ufl.edu/hansen/Protocols/Sexing%...
    Added: Wed Feb 24 2010, Hits: 88, Reviews: 0  Write review  
  • Simple protocol for differential staining of inner cell mass and trophectoderm of bovine embryos PDF file (PJ Hansen Laboratory)
    A simple protocol for determining trophectoderm and inner cell mass cells
    http://www.animal.ufl.edu/hansen/Protocols/Block,%...
    Added: Thu Oct 01 2009, Hits: 160, Reviews: 0  Write review  
  • TUNEL procedure for preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
    A protocol for detecting cell death using TUNEL.
    http://www.animal.ufl.edu/hansen/protocols/tunel.h...
    Added: Tue May 14 2002, Hits: 568, Reviews: 0  Write review   Cached