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Protocols
- Lab Protocols in Developmental Biology (Experiments in Developmental Biology)
A collection of protocols on Sea Urchin, Zebrafish, amphibians (Xenopus & axolotl), chick,and mouse
http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/D...
Added: Wed Mar 04 2009, Hits: 1078, Reviews: 0 Write review Cached -
Cell number counting in preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number
http://www.animal.ufl.edu/hansen/protocols/embryoc...
Added: Tue May 14 2002, Hits: 1188, Reviews: 0 Write review Cached -
Immunolocalization of CDX2 in Bovine Embryos – A Method for Differential Counting of Trophectoderm a
(PJ Hansen Laboratory, University of Florida)
For the current procedure, we take advantage of the fact that the transcription factor CDX2 is localized exclusively to trophectoderm (TE) to perform differential cell counting. Cells that are labeled with antiCDX2 and a nuclear dye (here DAPI but others can be used also) are considered TE whereas labeled nuclei that are negative for CDX2 are considered inner cell mass. The immunodetction method can be used for other antigens also.
http://www.animal.ufl.edu/hansen/Protocols/Immunod...
Added: Wed Jun 15 2011, Hits: 231, Reviews: 0 Write review -
In Vitro Production of Bovine Embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos
http://www.animal.ufl.edu/hansen/IVF/default.htm
Added: Tue May 14 2002, Hits: 899, Reviews: 0 Write review Cached -
Lineage Analysis Using Retrovirus Vectors
(Cepko Lab, Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute,)
Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.
http://axon.med.harvard.edu/~cepko/lineage/
Added: Tue May 14 2002, Hits: 507, Reviews: 0 Write review Cached -
Mini-Chamber for regulating gaseous environment during culture
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device
http://www.animal.ufl.edu/hansen/protocols/minigas...
Added: Tue May 14 2002, Hits: 694, Reviews: 0 Write review Cached -
Sexing Bovine Preimplantation Embryos by PCR
(PJ Hansen Laboratory)
a simple PCR based protocol for distinquishing between male and female embryos
http://www.animal.ufl.edu/hansen/Protocols/Sexing%...
Added: Wed Feb 24 2010, Hits: 498, Reviews: 0 Write review -
Simple protocol for differential staining of inner cell mass and trophectoderm of bovine embryos
(PJ Hansen Laboratory)
A simple protocol for determining trophectoderm and inner cell mass cells
http://www.animal.ufl.edu/hansen/Protocols/Block,%...
Added: Thu Oct 01 2009, Hits: 510, Reviews: 0 Write review -
TUNEL procedure for preimplantation embryos
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
A protocol for detecting cell death using TUNEL.
http://www.animal.ufl.edu/hansen/protocols/tunel.h...
Added: Tue May 14 2002, Hits: 991, Reviews: 0 Write review Cached