Summary: Metabolic labeling of cells with low-energy beta-emitting radioisotopes such as [35S]methionine is often used to follow the biosynthesis, maturation, and degradation of proteins in vivo.
Protocols
- Metabolic Labeling Using 32P (Mcmanus Lab, UCSF)
Protocol for metabolic labeling with orthophosphate.
http://mcmanuslab.ucsf.edu/node/108
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Metabolic Labeling
(Cowin Lab, New York University Medical Center)
Labeling cells with 35S-Met
http://celljunctions.med.nyu.edu/lab/notprivate/me...
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Metabolic labeling & Immunoprecipitation
(Springer Lab, Harvard University)
http://labs.idi.harvard.edu/springer/protocols/jun...
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Metabolic Labeling of Cells with 35S
(The Bjorkman Group at Caltech)
http://www.its.caltech.edu/~bjorker/Protocols/Meta...
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