Summary: Metabolic labeling of cells with low-energy beta-emitting radioisotopes such as [35S]methionine is often used to follow the biosynthesis, maturation, and degradation of proteins in vivo.
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Protocols
- Metabolic Labeling Using 32P (Mcmanus Lab, UCSF)
Protocol for metabolic labeling with orthophosphate.
http://mcmanuslab.ucsf.edu/node/108
Added: Sun Oct 25 2009, Hits: 318, Reviews: 0 Write review Cached -
Metabolic Labeling
(Cowin Lab, New York University Medical Center)
Labeling cells with 35S-Met
http://celljunctions.med.nyu.edu/lab/notprivate/me...
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Metabolic labeling & Immunoprecipitation
(Springer Lab, Harvard University)
http://labs.idi.harvard.edu/springer/protocols/jun...
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Metabolic labeling of cell proteins with [35S]-met
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research)
http://hypertension.ucsd.edu/list_of_protocols/11_...
Added: Sat Mar 27 2010, Hits: 277, Reviews: 0 Write review Cached -
Metabolic Labeling of Cells with 35S
(The Bjorkman Group at Caltech)
http://www.its.caltech.edu/~bjorker/Protocols/Meta...
Added: Sun Oct 25 2009, Hits: 329, Reviews: 0 Write review