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Protocols
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Basic Method for Direct Immunofluorescence Labeling
(Iowa State University, Flow Cytometry Facility)
This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached.
http://www.biotech.iastate.edu/facilities/CELLHYB/...
Added: Sat Mar 11 2006, Hits: 3964, Reviews: 0 Write review Cached -
Basic Method for Indirect Immunofluorescence Labeling
(Iowa State University, Flow Cytometry Facility)
This is the method for indirect immunofluorescence labeling; that is, the antibodies do not have the fluorescent dye attached.
http://www.biotech.iastate.edu/facilities/CELLHYB/...
Added: Sat Mar 11 2006, Hits: 2623, Reviews: 0 Write review Cached -
Cell sorting by FACS
(Jim Mullins Laboratory, University of Washington)
Separation of T-Cell Subsets by FACStar
http://ubik.microbiol.washington.edu/protocols/fac...
Added: Thu Jul 10 2008, Hits: 855, Reviews: 0 Write review -
Direct Flow Cytometry (FACS) Protocol
(Abcam)
http://www.abcam.com/ps/pdf/protocols/direct_flow(...
Added: Sat Feb 11 2006, Hits: 8593, Reviews: 0 Write review -
Direct Staining Protocol for Flow Cytometry
(Millipore)
http://www.millipore.com/immunodetection/id3/flowc...
Added: Wed Feb 13 2008, Hits: 1212, Reviews: 0 Write review Cached -
Flow Cytometry: Indirect Immunofluorescence Staining of Human Platelets
(BD Pharmingen)
Protocol for general use.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Sat Jul 27 2002, Hits: 3487, Reviews: 0 Write review Cached -
Flow Sorting Fibroblasts Expressing GFP
(Fero Lab, Fred Hutchinson Cancer Research Center)
Detecting and sorting GFP positive cells by FACS
http://www.fhcrc.org/science/labs/fero/Protocols/F...
Added: Thu Jul 10 2008, Hits: 420, Reviews: 0 Write review -
Fluorescein Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/flabel.html
Added: Tue May 14 2002, Hits: 3384, Reviews: 0 Write review Cached -
Indirect Flow Cytometry (FACS) Protocol
(Abcam)
http://www.abcam.com/ps/pdf/protocols/indirect_flo...
Added: Sat Feb 11 2006, Hits: 4635, Reviews: 0 Write review -
Intracellular Antigen Staining
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
staining intracellular antigen for FACS analysis
http://cyto.mednet.ucla.edu/Protocols/intracel.htm
Added: Sat Feb 11 2006, Hits: 3029, Reviews: 0 Write review Cached