Protocols
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The Cell Environment (including types of culture medium) (Sigma)
In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. A good introduction here to the purpose and types of various of media, supplements.
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Sun Jul 04 2004, Hits: 13191, Reviews: 0 Write review Cached -
Basics on L-Glutamine in Cell Culture
(Sigma)
L-glutamine is an essential amino acid and is required by all mammalian cells in culture. The chemical attributes and functions of L-glutamine in cell culture are described.
http://www.sigmaaldrich.com/life-science/cell-cult...
Added: Mon Nov 03 2008, Hits: 78, Reviews: 0 Write review -
Can you autoclave liquid media?
(Invitrogen)
http://invitrogen.custhelp.com/cgi-bin/invitrogen....
Added: Thu Aug 29 2002, Hits: 3907, Reviews: 0 Write review Cached -
Cell Culture Media and Solutions
(Donis-Keller lab)
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc11.h...
Added: Tue May 14 2002, Hits: 10516, Reviews: 0 Write review Cached -
Components and Supplementation of Various Culture Media
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
Mostly ES culture media.
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/c...
Added: Sun Jul 28 2002, Hits: 5012, Reviews: 0 Write review Cached -
Fetal bovine serum vs. Fetal calf serum
(Invitrogen)
What is the difference between fetal bovine serum and fetal calf serum?
http://invitrogen.custhelp.com/cgi-bin/invitrogen....
Added: Thu Aug 29 2002, Hits: 8286, Reviews: 0 Write review Cached -
Heat Inactivation of Fetal Bovine Serum
(The Donelson Lab, University of Iowa)
Heat inactivating fetal calf serum
http://www.biochem.uiowa.edu/donelson/Database%20i...
Added: Tue Apr 26 2005, Hits: 3619, Reviews: 0 Write review Cached -
primary human keratinocytes (PHKs) raft culture media
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/cellcult/raftmed.html
Added: Fri Sep 06 2002, Hits: 913, Reviews: 0 Write review Cached -
Recipes for Tissue Culture Selective Drug Stocks
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/d...
Added: Sun Jul 28 2002, Hits: 1686, Reviews: 0 Write review Cached -
Serum Thawing & Heat Inactivation
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
How to thaw serum and how to heat inactivate serum
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Mon Oct 28 2002, Hits: 2003, Reviews: 0 Write review Cached