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Protocols
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Cleaning mammalian cells from yeast
(Adapted from Gibco-BRL)
simple and affordable protocol that works. No expensive reagents required. I met the same protocol at Gibco-BRL web site few years ago. After Gibco merged with Invitrogen this page was removed. I keep this protocol on my website since I experienced my first yeast contamination.
http://www.geocities.com/mesentsev/Biblioteque/dec...
Added: Thu Apr 15 2004, Hits: 4790, Reviews: 0 Write review Cached -
How do I decontaminate my tissue culture?
(Invitrogen)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contamination is bacteria, fungus, mycoplasma...
http://invitrogen.custhelp.com/cgi-bin/invitrogen....
Added: Thu Aug 29 2002, Hits: 6717, Reviews: 0 Write review Cached -
Removal of Yeast Contamination from Lymphoblast Cultures
(Donis-Keller lab)
This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc8.html
Added: Tue May 14 2002, Hits: 1655, Reviews: 0 Write review Cached -
Understanding And Managing Cell Culture Contamination
(Corning)
A must read if you routinely work with cell culture.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 7357, Reviews: 0 Write review