Protocols
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Cell Culture Quality Control Considerations (Sigma)
Covers the main areas of quality control that are of concern for tissue culture including: The quality of the reagents and materials The provenance and integrity of the cell lines The avoidance of microbial contamination
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Sun Jul 04 2004, Hits: 8753, Reviews: 0 Write review Cached - Mycoplasma Detection using DNA Staining
(Corning Life Science)
This 3-page protocol provides a detailed procedure for using Hoechst stain #33258 to stain the DNA in cell monolayers for detecting mycoplasmas or other prokaryotic organisms.
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 4111, Reviews: 0 Write review -
Authentication of Cell Lines
(Sigma)
How to know the cell you are working on is authentic? Here are some techniques used for authentication of cell lines such as iso-enzyme analysis, DNA fingerprinting, multi locus DNA fingerprinting and multiplex - PCR (STR) DNA profiling.
http://www.sigmaaldrich.com/Area_of_Interest/Life_...
Added: Sun Jul 04 2004, Hits: 1955, Reviews: 0 Write review Cached -
Cleaning mammalian cells from yeast
(Adapted from Gibco-BRL)
simple and affordable protocol that works. No expensive reagents required. I met the same protocol at Gibco-BRL web site few years ago. After Gibco merged with Invitrogen this page was removed. I keep this protocol on my website since I experienced my first yeast contamination.
http://www.geocities.com/mesentsev/Biblioteque/dec...
Added: Thu Apr 15 2004, Hits: 5958, Reviews: 0 Write review Cached -
How do I decontaminate my tissue culture?
(Invitrogen)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contamination is bacteria, fungus, mycoplasma...
http://invitrogen.custhelp.com/cgi-bin/invitrogen....
Added: Thu Aug 29 2002, Hits: 11478, Reviews: 0 Write review Cached -
Removal of Yeast Contamination from Lymphoblast Cultures
(Donis-Keller lab)
This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc8.ht...
Added: Tue May 14 2002, Hits: 2941, Reviews: 0 Write review Cached -
Understanding And Managing Cell Culture Contamination
(Corning Life Science)
This 23-page Corning Technical Guide examines the problems and causes of culture loss due to contamination and explores some of the important strategies for preventing these losses by careful culture management.
http://www.corning.com/Lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 9284, Reviews: 0 Write review