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Protocols

• (Protocol for an orthotopic renal cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/renal.aspx
  Added: Sat Dec 11 2010, Hits: 231, Reviews: 0  Write review  
• Mouse Orthotopic Bladder Cancer Model
  In this straightforward procedure, bladder tumors are established in female C57 mice through the use of catheterization, local cauterization, and subsequent cell adhesion. After their bladders are transurethrally catheterized and drained, animals are again catheterized to permit insertion of a platinum wire into bladders without damaging the urethra or bladder. The catheters are made of Teflon to serve as an insulator for the wire, which will conduct electrical current into the bladder to create a burn injury. An electrocautery unit is used to deliver 2.5W to the exposed end of the wire, burning away extracellular layers and providing attachment sites for carcinoma cells that are delivered in suspension to the bladder through a subsequent catheterization. Cells remain in the bladder for 90 minutes, after which the catheters are removed and the bladders allowed to drain naturally. The development of tumor is monitored via ultrasound. Specific attention is paid to the catheterization technique in the accompanying video.
  http://www.jove.com/Details.stp?ID=2535
  Added: Sat Feb 26 2011, Hits: 148, Reviews: 0  Write review  
• Orthotopic Model of Brain Tumor in Mouse (MouseCancerModel.com)
  http://mousecancermodel.com/braintumor.aspx
  Added: Sat Dec 11 2010, Hits: 199, Reviews: 0  Write review  
• Orthotopic Model of Uterine Cancer in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/uterine.aspx
  Added: Sat Dec 11 2010, Hits: 94, Reviews: 0  Write review  
• Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells (JoVE)
  This video protocol describes a method in which firefly luciferase-tagged human MPNST cells are orthopically xenografted into the sciatic nerve of immunodeficient mice.
  http://www.jove.com/details.php?id=2558
  Added: Sat Apr 23 2011, Hits: 86, Reviews: 0  Write review  
• Protocol for a bone metastasis cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/bonemeta.aspx
  Added: Sat Dec 11 2010, Hits: 176, Reviews: 0  Write review  
• Protocol for an orthotopic bladder cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/bladder.aspx
  Added: Sat Dec 11 2010, Hits: 97, Reviews: 0  Write review  
• Protocol for an orthotopic bone cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/bone.aspx
  Added: Sat Dec 11 2010, Hits: 102, Reviews: 0  Write review  
• Protocol for an orthotopic hepatic/liver cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/liver.aspx
  Added: Sat Dec 11 2010, Hits: 235, Reviews: 0  Write review  
• Protocol for an orthotopic prostate cancer model in mouse (MouseCancerModel.com)
  http://mousecancermodel.com/prostate.aspx
  Added: Sat Dec 11 2010, Hits: 206, Reviews: 0  Write review