Summary: In vivo imaging is a novel noninvasive technique for imaging biological processes in live animals and can provide insight into living organisms and helps to understand metabolic processes and disease related changes in the body. There are two commonly used in vivo imaging methods including bioluminescence imaging (BLI) and biofluorescence imaging (BFI), which enable monitoring of gene expression or disease progression in living organisms.
- In Vivo Luciferin Imaging Procedure (Caliper)
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribute in awake animals for about 5-15 minutes. The mice are placed into a clear Plexiglas anesthesia box (2.5-3.5% isofluorane) that allows unimpeded visual monitoring of the animals; e.g. one can easily determine if the animals are breathing...
Added: Wed Feb 04 2009, Hits: 2084, Reviews: 0
Bioluminescence Imaging of Mice
protocol for bioluminescent optical imaging of HO-1-luciferase transgenic mice provides a rapid in vivo assay of the upregulation of the heme oxygenase-1 (HO-1) gene expression in response to the Gua Sha procedure. HO-1 has long been known to provide cytoprotection against oxidative stress.
Added: Sat Mar 20 2010, Hits: 1090, Reviews: 0
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
Tumor growth can be monitored for several days by bioluminescence before the tumor size becomes palpable or measurable by traditional physical means. This rapid monitoring can provide insight into early events in tumor development or lead to shorter experimental procedures. Quantitative imaging of tumor growth using IVIS provides precise quantitation and accelerates the experimental process to generate results.
Added: Sat Mar 20 2010, Hits: 821, Reviews: 0
Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
Introduces basic procedures of mouse MRI/MRS experiments
Added: Sat Mar 20 2010, Hits: 663, Reviews: 0
Preparation of Luciferin for In Vitro and In Vivo Bioluminescent Assays
Luciferin is a substrate for luciferase and is injected into animals before in vivo imaging
Added: Wed Feb 04 2009, Hits: 1350, Reviews: 0
(Fero Lab, Fred Hutchinson Cancer Research Center)
Describes methods for performing in vivo animal imaging including anesthesia and acquiring images.
Added: Sun Feb 08 2009, Hits: 940, Reviews: 0