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Protocols
  • Analysis of murine bone marrow: specimen choice, sampling and processing (Giorgio Cattoretti, MD, Columbia University )
    Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimental hematology focuses on femur and tibia as the source of BM. However, the thickness of the cortical bone and the often preferred use of the largest bone, the femur, for cell culture or flow leaved to the pathologist a hard specimen with little marrow. Nice protocol with photos.
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
    Added: Sat Jul 27 2002, Hits: 2092, Reviews: 0  Write review   Cached
  • Decalcification of Animals PDF file (Dr. Ornitz Lab, Washington University in St. Louis)
    http://molecool.wustl.edu/ornitzlab/PDFs/Protocols...
    Added: Tue Jul 11 2006, Hits: 645, Reviews: 0  Write review  
  • Dissociation of spleen and hemopoietic tissue (Giorgio Cattoretti, MD, Columbia University )
    Dissociation of mouse cell
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
    Added: Sat Jul 27 2002, Hits: 1664, Reviews: 0  Write review   Cached
  • Harvesting peritoneal cells (Giorgio Cattoretti, MD, Columbia University )
    Surgical procedure for collecting mouse peritoneal cells
    http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
    Added: Sat Jul 27 2002, Hits: 4136, Reviews: 0  Write review   Cached
  • Histopathological Approach to Rat Liver Tissue PDF file (Protocol Online)
    Added: Tue Aug 26 2008, Reviews: 0  Write review  
  • Mouse Skeleton Preparation PDF file (Dr. Ornitz Lab, Washington University in St. Louis)
    http://molecool.wustl.edu/ornitzlab/PDFs/Protocols...
    Added: Tue Jul 11 2006, Hits: 482, Reviews: 0  Write review  
  • Osteoblast Harvest from Mice (SurgeryResearch.com)
    Extract osteoblast cell from mice.
    http://www.surgeryresearch.com/Protocols/OsteoMice...
    Added: Wed Sep 25 2002, Hits: 1921, Reviews: 0  Write review   Cached
  • Processing mouse tissue (Giorgio Cattoretti, MD, Columbia University )
    • Routine dissection
    • Formalin fixation 
    • Storage of smears and slides 
    • Tissue embedding in paraffin

    http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
    Added: Sat Jul 27 2002, Hits: 4922, Reviews: 0  Write review   Cached