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SDS-sample buffer

Category: Buffers & Solutions
Author: Admin eLabJournal

Labels: SDS-PAGE

Materials

 

• 1--other--M Tris-HCl (pH 6.8)
• Sodium dodecyl sulfate (SDS)
• glycerol (87%)
• 0.5--other--M EDTA (pH 8.0)
• Bromophenol blue

Experiment settings

Volume SDS-sample buffer	5102550--other--ml
Concentration fold:	24--other--X

Step 1

Prepare --other--ml  of SDS-sample buffer --other--X by adding:

• --other--ml  Tris-HCl (pH 6.8)
• --other--g  SDS
• --other--ml  glycerol (87%)
• --other--ml  EDTA (pH8.0)
• --other--mg  bromophenol blue
• --other--ml  ß-mercaptoethanol

Step 2

Add --other--ml  H₂O to make a total volume of --other--ml  and mix well

Step 3

Make aliquots and store at -20ºC

• DTT may be added instead of ß mercaptoethanol as a reducing agent.

 

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