Category: Buffers & Solutions
Author: Admin eLabJournal
Labels: SDS-PAGE
Materials
Experiment settings
Volume SDS-sample buffer | ml | |
Concentration fold: | X | |
Step 1
Prepare
ml of SDS-sample buffer
X by adding:
- ml Tris-HCl (pH 6.8)
- g SDS
- ml glycerol (87%)
- ml EDTA (pH8.0)
- mg bromophenol blue
- ml ß-mercaptoethanol
Step 2
Add ml H2O to make a total volume of ml and mix well
Step 3
Make aliquots and store at -20ºC
- DTT may be added instead of ß mercaptoethanol as a reducing agent.