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SDS-sample buffer - eLabJournal.com
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Category: Buffers & Solutions
Author: Admin eLabJournal

Labels: SDS-PAGE

Materials
 

Experiment settings

Volume SDS-sample buffer ml   
Concentration fold:  

Step 1
Prepare ml  of SDS-sample buffer by adding:
  • ml  Tris-HCl (pH 6.8)
  • SDS
  • ml  glycerol (87%)
  • ml  EDTA (pH8.0)
  • mg  bromophenol blue
  • ml  ß-mercaptoethanol
Step 2
Add ml  H2O to make a total volume of ml  and mix well
Step 3
Make aliquots and store at -20ºC
  • DTT may be added instead of ß mercaptoethanol as a reducing agent.