Archived Protocol

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class="materials"><table><tbody><tr><td style="border: medium none;">Tryptone</td><td style="border: medium none;"><br></td></tr><tr><td style="border: medium none;">Yeast extract<br></td><td style="border: medium none;"><br></td></tr><tr><td style="border: medium none;">NaCl<br></td><td style="border: medium none;">58.44 g mol<sup>-1</sup><br></td></tr></tbody></table></div> <span class="break"></span> <div id="experimentsettingsbar"> <h2 class="titlebar">Experiment settings</h2> <div class="experimentsettings"><table> <tbody> <tr> <td style="border: medium none;"> <strong>Volume of LB medium:</strong></td> <td style="border: medium none;"> <select id="VolLB" name="VolLB" onchange="da('VolLB');fvarFnVolLB('VolLB',Ext.fly(this).getValue());"><option value="1">1</option><option value="2">2</option><option value="5">5</option><option value="@@--@@">--other--</option></select><span class="ej_unit">L </span></td> </tr> </tbody> </table> <br> </div> <span class="break"></span> </div> <div id="step_0"><h6 class="step">Step 1</h6><div class="stepcontent">Prepare <select id="VolLB_copy" name="VolLB_copy" onchange="da('VolLB_copy');fvarFnVolLB_copy('VolLB_copy',Ext.fly(this).getValue());"><option></option><option value="@@--@@">--other--</option></select><span class="ej_unit">L </span> of LB medium by adding:<br> <ul> <li> <select id="MassTryp" name="MassTryp" onchange="da('MassTryp');fvarFnMassTryp('MassTryp',Ext.fly(this).getValue());"><option></option><option value="@@--@@">--other--</option></select><span class="ej_unit">g </span> Tryptone</li> <li> <select id="MassYeast" name="MassYeast" onchange="da('MassYeast');fvarFnMassYeast('MassYeast',Ext.fly(this).getValue());"><option></option><option value="@@--@@">--other--</option></select><span class="ej_unit">g </span> Yeast extract</li> <li> <select id="MassNaCl" name="MassNaCl" onchange="da('MassNaCl');fvarFnMassNaCl('MassNaCl',Ext.fly(this).getValue());"><option></option><option value="@@--@@">--other--</option></select><span class="ej_unit">g </span> NaCl</li> </ul> </div></div><div id="step_1"><h6 class="step">Step 2</h6><div class="stepcontent">Add dH<sub>2</sub>O until the total volume of <br> </div></div><div id="step_2"><h6 class="step">Step 3</h6><div class="stepcontent">Set to pH 7 by adding one or two drops of NaOH<br> </div></div><div id="step_3"><h6 class="step">Step 4</h6><div class="stepcontent">Make aliquots and autoclave<br> </div></div></table> <div class="warning" id="notes" style="visibility:hidden;">[Notes]</div> </div> <input type="hidden" name="initpdf" id="initpdf" value="false" /> <input type="hidden" name="id" value="29" /> </div> </form> <div id="ctl00_TemplateBody_PanelCommentLogin"> <h1><a href="/login/?redir=%2fviewer%2f?id=29" class="btn">Please login to comment</a>Comments</h1> </div> <a name="comments"> </a> <script type="text/javascript"> var fvarFnVolLB = function(varID,varValues){ if (ex('VolLB_copy')) {setValue('VolLB_copy', ( ROUNDDEC(P(Ext.fly('VolLB').getValue()),2) ));} if (ex('VolLB_copy')) {fvarFnVolLB_copy();} //{VolLB} }; var fvarFnVolLB_copy = function(varID,varValues){ if (ex('MassNaCl')) {setValue('MassNaCl', ( ROUNDDEC(P(Ext.fly('VolLB_copy').getValue())*10,2) ));} if (ex('MassNaCl')) {fvarFnMassNaCl();} if (ex('MassTryp')) {setValue('MassTryp', ( ROUNDDEC(P(Ext.fly('VolLB_copy').getValue())*10,2) ));} if (ex('MassTryp')) {fvarFnMassTryp();} if (ex('MassYeast')) {setValue('MassYeast', ( ROUNDDEC(P(Ext.fly('VolLB_copy').getValue())*5,2) ));} if (ex('MassYeast')) {fvarFnMassYeast();} //{VolLB_copy} }; var fvarFnMassTryp = function(varID,varValues){ //{MassTryp} }; var fvarFnMassYeast = function(varID,varValues){ //{MassYeast} }; var fvarFnMassNaCl = function(varID,varValues){ //{MassNaCl} }; var ej_varReg = new Array('VolLB','VolLB_copy','MassTryp','MassYeast','MassNaCl'); var ej_funcReg = new Array('fvarFnVolLB','fvarFnVolLB_copy','fvarFnMassTryp','fvarFnMassYeast','fvarFnMassNaCl'); 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