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ChIP Related Discussions
- parameters for normalisation in ChIP - how to represent ChIP data (reply: 2)
- Primer design for PCR following ChIP - (reply: 3)
- losing the signals in ChIP - (reply: 2)
- Will salmon sperm DNA in ChIP protocol interfere the WGA amplification? - (reply: 1)
- qPCR problems with chIP samples - (reply: 2)
- Phenol/chloroform vs Column purification for ChIP DNA - (reply: 8)
- Advantages of ChIP vs. sodium bisulfite sequencing - (reply: 2)
- Nimblegen Chip on Chip Data analysis - Data analysis (reply: 2)
- Yield after ChIP Assay - (reply: 6)
- CHIP - only negative results! - (reply: 6)
- ChIP for Dummies - (reply: 9)
- ChIP Input (control) necessity - (reply: 1)
- Inconsistent ChIP PCR - PCR bands vary from same DNA sample (reply: 3)
- ChIP for virus - (reply: 5)
- ChIP sonification woes - why is my DNA cut into tiny pieces?? :( (reply: 7)
- Negative Control Rabbit IgG for ChIP Assay - (reply: 3)
- problem with WGA amplification of my chip sample - (reply: 4)
- Chip Troubles - Help: Strong PCR signal from negative control region (reply: 7)
- ChIP on chip - simple questions (reply: 1)
- Finding out what sequence to study by ChIP - (reply: 21)
- Low Ct value in ChIP qPCR - (reply: 4)
- Removing RNA from DNA during ChIP Assay - (reply: 3)
- CHIP / Is it normal to see pellet after the DNA purification? - (reply: 3)
- Best Antibody for CHiP (H3K4 and H3K27) - Looking for the best antibody for histone CHiP (reply: 1)
- Chromatin IP - What BSA to use for ChIP (reply: 1)
- Can anyone recommend a good IgG Ab for negative control for ChIP? - (reply: 2)
- Crazy ChIP! Background probs and All the sudden no signal! - (reply: 3)
- which qiagen kit could be used in DNA recovery in ChIP assay? - (reply: 6)
- which array platform fro ChIP -chip + /-exaction program you prefer - sharing your ChIP-chip experience on TF and Histone modification (reply: 1)
- monoclonal antibody or polyclonal antibody? - which is better for ChIP? (reply: 1)
- Question about sonication step for ChIP assay - (reply: 5)
- CHIP : Use frozen tissue - (reply: 1)
- Problem with qPCR of ChIP input - (reply: 12)
- ChIP sonication:different smear in cross-linked and non-corsslinked samples - (reply: 8)
- ChIP: SDS precipitates during sonication...any ideas? - (reply: 7)
- i was confused about ChIP result - i need help (reply: 1)
- Labelling ChIP samples - ARG! Hope someone can help! (reply: 1)
- ChIP and qPCR - ChIP and qPCR (reply: 4)
- Input problem with fast chip protocol - (reply: 6)
- ChIP densitometry - (reply: 1)
- control for ChIP - (reply: 2)
- input problem from ChIP prep - (reply: 5)
- Worries about ChIP results.. - (reply: 1)
- some questions about fast ChIP - (reply: 2)
- High quality formaldehyde for ChIP? - (reply: 2)
- Nuclear extraction of brain tissue for ChIP - (reply: 3)
- No DNA after sonication for ChIP - (reply: 2)
- ChIP sample storage - (reply: 4)
- ChIP Fixation & Protease inhibitors - (reply: 1)
- ChIp on mouse liver - (reply: 9)
- ChIP and qRT-PCR - (reply: 1)
- Fast ChIP Method and Chelex - (reply: 5)
- ChIP Troubleshoot - (reply: 1)
- Chip Assay - PCR step (reply: 3)
- ChIP qPCR data analysis? - (reply: 9)
- Magna ChIP kit from Upstate - Insufficient volume or reagents? (reply: 1)
- chip assay primers - chip assay primers (reply: 2)
- CHIP enrichment varies? anyone can help? - (reply: 3)
- ChIP on chip - can anyone help me? - (reply: 4)
- Magnetic ChIP - Magnetic Rack - (reply: 4)
- ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
- ChIP - Is it possible to spin beads too hard? - (reply: 8)
- How do you determine the linear range of ChIP and input samples? - (reply: 2)
- ChIP sonication problem - (reply: 7)
- Affymetrix chip - unexpressed genes, which signal cuttof to use - (reply: 1)
- no-antibody control is stronger than my sample! - in ChIP.. (reply: 5)
- Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
- ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
- RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
- ChIP on peripheral blood samples.... - anyone done it?? (reply: 2)
- ChIP: bright 300bp band after RNAse A - (reply: 1)
- Help: RNaseA or Proteinase K or both for DNA extraction in CHIP - (reply: 3)
- ChIP PCR problems - I can't get rid of the smears! - (reply: 6)
- CHIP calculation. confused! - (reply: 2)
- QPCR versus regular PCR for Chip - (reply: 2)
- ChIP has eaten all my DNA! - (reply: 1)
- good positive controls for Chip - (reply: 3)
- why do I see enrichment in ChIP inner control? - (reply: 2)
- How to obtain pure DNA from ChIP - (reply: 3)
- ChIP assay using Matrigel-3D cell culture - (reply: 2)
- positive IgG in ChIP - (reply: 2)
- ChIP - reproducibility problem - (reply: 9)
- what is meaning of true enrichment for chip qPCR? - (reply: 5)
- What in ChIP samples inhibit PCR? - (reply: 2)
- chip assay dna pptn - (reply: 3)
- ChIP'ing for Big, Indirect Coactivators - Looking for tips on how to get this difficult ChIP to work.. (reply: 6)
- Designation of PCR primers in ChIP experiment - (reply: 3)
- CHIP western - Western after CHIP (reply: 4)
- ChIP chip stripping - (reply: 1)
- PCR smear with CHIP - (reply: 2)
- Smear in PCR after ChIP - (reply: 2)
- how to purify ChIP DNA by using kit - (reply: 4)
- How many cycles in PCR would you recommend for ChIP - (reply: 3)
- ChIP with unknown DNA target - (reply: 2)
- ChIP and qPCR calculation ? - (reply: 8)
- Yeast ChIP Help - No DNA after sonication - - (reply: 2)
- Is this sonication result good for ChIP? - (reply: 6)
- ChIP PCR help, maybe is the problem of SDS. - (reply: 4)
- chromatin yield (ChIP) - chromatin yield from primary cells (reply: 3)
- Working antibody for human NFkB (p65) ChIP assay ? - (reply: 2)
- Input Control for ChIP - (reply: 3)
- what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
- Filamentous pellet when you sonicate in ChIP assay - (reply: 2)
- solid support for ChIP washes - (reply: 7)
- ChIP tag, Flag or HA sequence - (reply: 3)
- Companies for ChIP Ab - (reply: 2)
- ChIP IgG has the same signal than the antibody tested - (reply: 9)
- ChIP on chip Affymetrix - Ampification protocol - Quality of amplified DNA (reply: 5)
- Controls in ChIP experiments? - (reply: 5)
- ChIP DNA concentration - (reply: 7)
- CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
- Negative control primer for ChIP - (reply: 3)
- Using Westerns to check crosslinking in ChIP - (reply: 2)
- ChIP _ genomic DNA isolation - (reply: 1)
- Post ChIP DNA amount - (reply: 1)
- My primers don't work with Chip - (reply: 1)
- how to validated CHIP after H3k27 3Me - (reply: 5)
- ChIP in mouse tissues, How to crosslink ? - (reply: 1)
- CHIP: No enrichment in IP DNA Relative to Input - (reply: 4)
- ChIP assay! - (reply: 1)
- Can restriction digestion be replaced by CHIP? - (reply: 5)
- Special Question to KPDE - Fast chIP (reply: 2)
- Protease inhibitors used in CHIP - (reply: 2)
- what is the perfect volume for immunoprecipitation step of ChIP? - (reply: 4)
- real time PCR with ChIP - (reply: 4)
- How do people shear their chromatin for ChIP? - Tips and Hints with Fragmenting Chromatin (reply: 1)
- CHIP sonication chromatin size - (reply: 1)
- ChIP: I get smear after PCR? - (reply: 2)
- ChIP on Affy's Human Promoter 1.0R array - (reply: 20)
- Why my ChIP PCR results are always unrepeatable? even from the same sample. - (reply: 7)
- Titrate chromatin for chIP - (reply: 2)
- Help with ChIP analysis using Taqman - (reply: 1)
- ChIP DNA yield problem - (reply: 2)
- No product after chIP? - (reply: 1)
- Selection of Antibodies for CHip - (reply: 7)
- Please Help me, about Chromatin IP(ChIP)! - CHIP (reply: 1)
- problem of DNA sonication in CHIP assay - (reply: 2)
- Please check my ChIP sonication gel - (reply: 4)
- Does anyone know any kind of array or chip for methylation scan? - (reply: 3)
- About two-step Chip - (reply: 1)
- testing CHIP samples on Western - how to de-crosslink (reply: 2)
- ChIP assay results interpretations - (reply: 3)
- Is ChIP is a good choice to find the DNA sequence for a binding protein? - (reply: 2)
- ChIP and CpG island microarrays - (reply: 1)
- Dynal (Magnetic) beads for ChIP? - (reply: 3)
- ChIP - (reply: 1)
- ChIP sonication problem - (reply: 4)
- How to purify the ChIP DNA - (reply: 4)
- ChIP assay - (reply: 2)
- problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
- My ChIP product Western is dirty - (reply: 7)
- Has anyone read about doing ChIP on tumor specimans? - (reply: 1)
- why couldn't I see my protein on western blot after ChIP? - (reply: 3)
- The Histone H3.1 and Histone H3.3 variant quandry - How can you tell the difference in a ChIP?? (reply: 2)
- ChIP Assay and no clear answer from professor - (reply: 3)
- ChIP on frozen tissue samples - (reply: 7)
- ChIP: Equal DNA Pull-Down in Positive and Negative Controls - HELP - (reply: 14)
- ChIP from low amount of cells - (reply: 1)
- Problem in first step in ChIP - (reply: 9)
- ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
- ChIP control for transcriptional repressor? - (reply: 1)
- How long can the whole cell extract for ChIP stay in -80C? - (reply: 1)
- EMSA done...what would ChIP assay add - (reply: 2)
- how to evaluate chIP enrichment efficency? - (reply: 1)
- ChIP crosslinking - (reply: 2)
- ChIP qPCR analysis - (reply: 2)
- Got different size PCR products from chIP - (reply: 7)
- How can I extract Chromatin-protein complex without using Chip? - The cheapest method to prove a protein to bind DNA (reply: 3)
- ChIP sonication problems...can I use 20 sec pulse? - (reply: 2)
- ChIP control problems - (reply: 1)
- how everyone obtain skills for chip assay? - Is it necessary to learn it from another lab? (reply: 5)
- positive controls for CHIP - (reply: 2)
- pre-immune serum in CHIP IP - (reply: 1)
- ChIP sonication problems - shearing dna before actually sonicating (reply: 3)
- How to design PCR primers in ChIP assay - (reply: 2)
- strange CHIP input result - (reply: 3)
- How to test ChIP cross-linking and reversal? - (reply: 18)
- ChIP sonication problem: too wide range of smear - too wide range of smear (reply: 9)
- how many antibody eveyone use for CHIP assay - (reply: 2)
- CHIP and CHIP with tagged proteins - (reply: 1)
- Advantages of ChIP over EMSA - (reply: 3)
- internal assay controls for histone ChIP - (reply: 6)
- ChIP protocol and tips - (reply: 8)
- function of LiCl in ChIP wash buffer - (reply: 1)
- CHIP - Harvesting cells by trypsin - (reply: 1)
- starting amount of DNA for ChIPs - using the ChIP for ChIP-on-chip analysis (reply: 1)
- HELP! Background in ChIP assay - (reply: 1)
- ChIP Normalization - (reply: 2)
- DNA shearing optimization for ChIP assay - (reply: 2)
- CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
- Cross-linking and cross-link reversal for ChIP - (reply: 3)
- ChIP quantification (qPCR) - (reply: 2)
- ChIP DNA purification - URGENT please (reply: 8)
- Nonspecific precipitation in ChIP - (reply: 4)
- ChIP assay sonication problem - (reply: 36)
- 6 Hour ChIP method - (reply: 3)
- ChIP analysis using tissue and controls - (reply: 3)
- ChIP - no immunoprecipitated DNA - (reply: 12)
- ChIP: Sonication - (reply: 7)
- ChIP protokol question - (reply: 2)
- DNA amount in ChIP - (reply: 2)
- Reliable ChIP primers for Sp1? - (reply: 2)
- CHIP on Chip array - (reply: 11)
- Has anyone tried to sonicate Jurkat cells for ChIP assay? - (reply: 7)
- ChIP sonication Input Agarose gel - (reply: 1)
- CHIP with Tagged Proteins - (reply: 2)
- help with ChIP control--urgent! - (reply: 6)
- Nuclear extracts for ChIP? - (reply: 1)
- Avoiding non-specific DNA binding in ChIP assays - (reply: 3)
- ChIP DNA sonication conditions - (reply: 3)
- Problematic results with ChIP - need help troubleshooting - (reply: 3)
- ChIp using Drossophila imaginal discs - (reply: 1)
- qPCR after ChIP - lack of reproducibility - (reply: 9)
- CHIP FOR CBP - (reply: 1)
- Blocking in ChIP - (reply: 2)
- MNase vs sonication for ChIP - (reply: 6)
- On antibody storage and ChIP - (reply: 3)
- affymetrix gene chip compering to agilent gene chip - (reply: 2)
- ChIP sonication using a cup horn - (reply: 1)
- Variability in ChIP assay - (reply: 4)
- Trascruz reagents for ChIP assays - (reply: 3)
- DNA length to be amplified in ChIP. - What should be DNA size range? (reply: 1)
- ChIP analysis - how to analyze CHIP data accuretly? - (reply: 2)
- Discussion: ChIP PCR control - (reply: 12)
- No PCR products with the ChIP DNA - (reply: 5)
- SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
- lightcycler controls and standard for CHIP - (reply: 3)
- Which antibody to use for ChiP? - Monoclonal or polyclonal? (reply: 5)
- Problems in CHIP assay? - How can i get rid of multiple bands in CHIP assay? (reply: 3)
- amount of DNA after Chip - (reply: 1)
- Can ChIP be applied on tissue samples? - (reply: 5)
- ChIP Negative Control - Chromatin Immunoprecipitation (reply: 1)
- ChIP lysis buffer - Chromatin (reply: 2)
- Using qiagen kit for ChIP DNA purification - (reply: 4)
- Sepharose or agarose in ChIP assay - (reply: 2)
- ChIP with primary human CD4+ Lymphocytes possible ? - (reply: 1)
- Q re: optimizing sonication in ChIP - (reply: 2)
- CHIP - region - (reply: 1)
- ChIP again... - (reply: 1)
- tissue ChIP - (reply: 2)
- Can I use the upstate ChIP protocol for mouce tissue? - (reply: 5)
- Background in CHIP - (reply: 7)
- USING MISONIX MICROSON FOR SONICATION FOR CHIP ANALYSIS - (reply: 3)
- ChIP sample storage - (reply: 5)
- Question about ChIP? - (reply: 2)
- ChIP - some basic FAQs - (reply: 9)
- CHIP with nuclei - (reply: 1)
- ChIP assay loading control - (reply: 6)
- ChIP DNA sonication problem - got too big and too small fragments (reply: 3)
- non-specific band in ChIP assay - (reply: 1)
- How to search and retrieve promoter sequence for methylation or ChIP study - (reply: 17)
- ChIP assay - Foaming in sonication and other woes - (reply: 41)
- Ampification in no-antibody control of ChIP assay - High background - (reply: 14)
- ChIP against transcription factors - (reply: 7)
- PCR for ChIP - PCR for ChIP not working (reply: 3)
- ChIP sonication woes - Losing DNA? (reply: 4)
- sonication for ChIP - protocols for sonication in a water bath for ChIP (reply: 2)
- ChIP Assay and sonicators - ChIP assay question (reply: 4)
- FLAG (M2) EZView beads in ChIP assay - Background binding trouble (reply: 4)
- ChIP assay - (reply: 2)
- CHIP assay - (reply: 1)