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Cytokine Expression Profiling
(eBioscience)
ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for a protein followed by an appropriate incubation period. Subsequently, the protein of interest is detected in the environment immediately surrounding the cell secreting it, with another antibody specific for a different epitope of the protein. The signal detected by the HRP enzyme/substrate results in a colorimetric footprint of the cells and can be quantitated by visual scoring or specialized plate-readers.
http://www.ebioscience.com/ebioscience/appls/ELISP...
Added: Tue May 14 2002, Hits: 674, Reviews: 1 Read reviews
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