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  • Cytokine Expression Profiling (eBioscience)
    ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. In the current technique, cells are deposited onto a membrane coated with one antibody specific for a protein followed by an appropriate incubation period. Subsequently, the protein of interest is detected in the environment immediately surrounding the cell secreting it, with another antibody specific for a different epitope of the protein. The signal detected by the HRP enzyme/substrate results in a colorimetric footprint of the cells and can be quantitated by visual scoring or specialized plate-readers.
    http://www.ebioscience.com/ebioscience/appls/ELISP...
    Added: Tue May 14 2002, Hits: 640, Reviews: 1 Read reviews  Write review   Cached

    • 11 of 54 people found the following review helpful:
    elispot, Sun Oct 27 2002
    Reviewed By: mohammad reza sam
    anti-hbs elispot assay
    elispot protocol for enumerating anti-hbs specific b lymphocyte

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