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  • MicroArray Protocol PDF file (HuGEIndex.org)
    Basic Steps of microArray experiments
    • Total RNA Isolation from cultured cells.
    • DNase Treatment of Total RNA
    • Making the single strand cDNA probe.
    • Automated Slide Processor (ASP) Version for hybridization.
    • Washing microarrays in ASP.
    • Processing of Array slide
    • Pre-hybridization of the processed slides (NON-Automated version).
    • Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).
    • Preparation of Dendrimer Cy3 and Cy5.
    • Washing unbound probe from glass array (NON-Automated version).
    • Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).
    • Washing unbound dendrimer from glass array(NON-Automated version).

    http://132.183.243.138/hio/microarray/microprocedu...
    Added: Tue Sep 24 2002, Hits: 6387, Reviews: 1 Read reviews  Write review  

    • 14 of 66 people found the following review helpful:
    MicroArray Protocol Using GraceBio CoverSlips, Thu Dec 19 2002
    Reviewed By: Bryant McLaughlin
    The gracebio sticky covers can be made to any size you want and thus accomodate whatever array size you have.

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