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HELP: adenovirus transfection - (Sep/30/2005 )

Hey,everyone

I'm going to use adenovirus to infect CD34+,293,K562 and CHO. Someone knows any protocol can i use?

Thanks!fengqiouhuang

fengqiouhuang@sina.com
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-fengqiouhuang-

hi
this protocol from standford may help you.
fred

-fred_33-

tongue.gif tongue.gif tongue.gif
fred 33 ,thanks!
But there are another problems, such as the transfection conditions, I hope that friends who working or worked in this feild could help me (your bright ideas or experiences), tongue.gif tongue.gif tongue.gif thank you a lot!
fengqiouhuang
fengqiouhuang@sina.com

-fengqiouhuang-

hi,everyone
use adenovirus to transfect CD34+,what conditions will be profect?
555555.....55,help me !
fengqiouhuang
thanks!

-fengqiouhuang-