Recommend a nice and handy program for sequence manipulation - A MS Word addin (Sep/23/2005 )

Muller PY, Studer E, Miserez AR.
Molecular Biocomputing Suite: a word processor add-in for the analysis and manipulation of nucleic acid and protein sequence data. Biotechniques. 2001 Dec;31(6):1306, 1308, 1310-3.

The MBCS software aims to manage and expedite a wide range of sequence analyses and manipulations using an integrated text editor environment including menu-guided commands. Its independence of sequence formats enables MBCS to be used as a pivotal application between other software tools for sequence analysis, manipulation, annotation, and editing.

To use it with Word, unzip the attached zip file you will get one file named mbcs.dot, put the file into
C:\Program Files\Microsoft Office\Office\Startup (depending on where you install MS office). Every time when you start Word, MBCS will lanuch automatically and you will see an additional menu item in Word. If you have problem running it in Word, maybe you have to lower the Macro security setting in Word.

Enjoy.

I also like to recommend a nice and free online program BioInfoMan at http://bioinfoman.com/bm/. Here are some of the unique tools from this program.

o Easy-to-use (the easiest!) tool to search and retrieve molecules from on-line databases.
o Powerful and foolproof cloning wizard: it also includes the gene synthetic cloning, TA cloning, D-TOPO cloning and Gateway cloning that are not available in any commercial products.
o One-click to design sequencing and PCR primers.
o Rare codon analysis of a sequence for any species.
o Optimized reverse translation:
• Codon optimization for any species
• GC content optimization
• Restriction site optimization
• RNA secondary structure optimization.
o Smart sequencing result analysis:
• Automatically trims the ambiguous sequences off the two ends of your results
• Automatically align reverse-complementary sequences
• Highlights the differences after alignment
• Aligns multiple sequencing results against a single target sequence and multiple input file formats are supported
• You can store your target sequences on-line (retrieved from GenBank or entered by yourself) and access them at any time.
o Auto-annotate constructs: By paste just sequences, the tool predicts all common features and converts your sequences to fully annotated GenBank format.
o Construct manipulation without losing annotation!
o Complete restriction digestion analysis.
o All types of protein and DNA sequence format conversion and manipulation: Users can do almost anything to manipulate the sequences, calculate the properties and so on.

hi,
i am alie from india...i have gone through the MBCS and i dowloaded it but i am not able to use it ....i dnt know much about bioinformatics...so could u help me out how to use this tool

thanx
alie

Hi

You can also try the free analyze sequence and plasmid creation tools at Addgene Labs. www.addgenelabs.org

All the common sequence analysis features are present, and you can use the system to create plasmid maps easily!