what is the difference of 293T, HEK293 and HEK293T? - (Sep/13/2005 )
I just get confused that so many adherent 293 cells. what is the difference of 293T, HEK293 and HEK293T? when will use one of them rather the other one?
293 and HEK293 cells are same
293T and HEK293T cells are same
293T cells are 293 cells transformed by expression of the large T antigen from SV40 virus that inactivates pRb.
you can get info on large T here
thanks a lot. does help lot
that's for such answers (but not only for that) why i love this forum
That is also why I like this forum.
by the way, normal 293 T cells whether can be cultured suspension?
No, they cannot be cultured in suspension... they will adhere to any plastic... and they need to be adherent to grow and divide.
That's not necessarily true. 293 cells may be successfully cultured in suspension, particularly in serum-free conditions. One such example of scale up of 293 using spinner flasks, which spin the cells in suspension, may be obtained here: http://www.ytbioteknik.uu.se/Center/BPU/se...ies/hek293.shtm
The earliest article about 293T I can find is Science, 1985, 277:23-28. Its original name is "293tsA1609neo". I can not get the full text, but I think it might be G418 resistant, according to the name.
According to "http://www.bio.net/bionet/mm/virology/2002-March/007480.html", 293T might be "exceptionally transfectible, more so than the parental 293 cells".