storing blood from mouse - Can blood be stored, and if yes, How? (Aug/26/2005 )
Hi,
I've got a question regarding storing blood obtained from mouse so that the red blood cells can be used the next day for binding studies. Currently, I'm unable to get viable cells for the binding assay if I store the blood overnight. Can someone please give me some good advice on how to go about handling and storing murine blood for use on another day? Sincere thanks!
ggUss
Many eons ago I used to perform CBC/chemistry at a diagnostic lab. We stored all of our blood at 4 degrees for up to one week in the event we needed to rerun something.
Whole blood is best stored with clotting inhibitor (ie. EDTA) in tube at 4 degrees. Don't freeze whole blood. Breakdown of RBCs will occur over time, so it is best to use ASAP. Serum or plasma can be frozen at -80 degrees until needed, but should be isolated immediately to prevent hemolysis.
On that note, hemolysis can also occur while obtaining the sample and handling it. I would consider spinning down one tube as a test to see if the hemolysis is due to sample prep or to the O/N storage.
Hope this helps.
Whole blood is best stored with clotting inhibitor (ie. EDTA) in tube at 4 degrees. Don't freeze whole blood. Breakdown of RBCs will occur over time, so it is best to use ASAP. Serum or plasma can be frozen at -80 degrees until needed, but should be isolated immediately to prevent hemolysis.
On that note, hemolysis can also occur while obtaining the sample and handling it. I would consider spinning down one tube as a test to see if the hemolysis is due to sample prep or to the O/N storage.
Hope this helps.
Hi,
Is the above also valid for blood from mouse? human blood can be stored for extended periods if stored appropriately . what about mouse blood? has anyone been using stored RBCs from mouse blood after storage overnight? Thanks.