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UV and nematodes - (Aug/24/2005 )

Hi! is anyone working with C elegans? it is my first time working with them and i need to irradiate them with UV. No one is working with them t my lab so i am trying to do everything from the beggining. i have a protocol for Psoralem mutagenesis and i know that is better to follow it as it is writting at the beggining but i was wondering if after washing them (the nematodes) with M9 Buffer it is necessary to suspend them in TMP in DMSO.I am also not sure why is this step for.



look at wormbook and read the section on microinjection they have a section of UV integration.