Is L-glutamine easily degradale in RPMI1640? - (Aug/17/2005 )

Is L-glutamine easily degradale in RPMI1640? if it is the case, I can not prepare a big volume for use each time!

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Hi,

we usually prepare a volume of 500ml medium with 5 ml L-glu, and everything is working well. It will depend how oft you use the medium. I will estimated I use the 500ml bottle for min one month. But we aliquot the L-glu (5-6ml) and froze them in order not to have to defroze everytime the stock solution.

Good luck
cuca

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Just to add to Cuca's advice, in the event that my media is still sitting there in the fridge after a month, I add half the initial volume of L-glu that was initially added to ensure that there is still some L-glu there (it does degrade after some time).

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You could also try glutamax, which is more stabile than L-glu.

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