other methods for the detection of antigens - (Aug/07/2005 )
as part of my assignment at grad skul, since i am new to immunology, i just want to ask about other techniques, or tests that i can use for the detection of antigens?
The best method for antigen detection is ELISA. For protocol there r plenty of websites available. Also u can use Westorn blotting .
Depends upon the ag. Small molecules; ie drugs (NIDA 5; for example) are required by law to be confirmed by GC MS(by FAA, DoT, etc)
hCG original test was with live animals.
Receptor assays for tumor markers (ie breast ca; ERA, PRA)
surface plasmon resonance
analysis of gene expression libraries with patients sera will give you ideas of up to date unkown antigens.