Hi,
I've got 2 drugs we're certain are acting by interferring with the Fas apoptotic pathway ONLY.

We need to check this by replacing one of these drugs with another Fas apoptotic pathway specific agent.

I thought I could just "buy" some fas ligand but I can only find antibodies for it, not the ligand itself.

Does anyone know if one can buy Fas Ligand or know of a drug that is cytotoxic specifically (or moderately specifically ) through the Fas/FADD apoptotic pathway?

Thanks

sigma-aldrich have FasL from humans and mouse:

http://www.sigmaaldrich.com/

Fas Ligand from Mouse (1)
F0552 >95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder (Sigma)

Fas Ligand human (1)
F0427 >95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder (Sigma)

Fas Ligand human (1)
F4428 >95%, recombinant, expressed in HEK-293 cells (amino acid residues 133-281, N-terminal FLAG tagged) (Sigma)

Fas Ligand, soluble human (1)
S8689 recombinant, expressed in human embryo kidney cells, lyophilized powder (Sigma),

for example.

mike

Right - the obvious sorce is NEVER the one you check LOL!!

Had managed to find one other company but it was in 'reserach production stage only"

Thanks

try with
SuperFasLigand™, Soluble (human) (recombinant)
http://www.axxora.com/APO (searching for FasL)

Seb_

Yeah I agree

SuperFasL works the best in our hands. Some of the commercial FLAGFasL constructs require cross linking with anti-FLAG antibodies to achieve the level of activity that SuperFasL has.


Good Luck