# confusion with the n-numbers - (Jun/21/2005 )

A silly question. Hope for ideas! Thanks in advance.

http://www.bioscience-events.com/leipzig/P...PCR-Leipzig.pdf

Page 16: what does it mean with n=6 in this context? Is that the number of different dilutions, runs or repeats of each different dilution?

Page 22 : What does it mean with n=1 in this context? Is that the number of different dilutions, runs or repeats of each different dilution?

hi

diapositive p 16 : n is the number of independant experiments. That says the experience was repeated 6 times and for each assay, there were 6measurments for each cell population.

p 21 : n is the number of experiments you've done to get results. if you did 1 exp, then n=1 (first formula). You take in count the measures of this exp. If you do several experiments (at least 2) then you take in count the average of the measures you did.

hope that helps...

fred