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Buffering systems - need ideas - (Apr/07/2005 )

Hello everybody,

When i use HEPES as the buffering system, does it affect my cells negatively if i incubate them in a CO2 gas incubator? This buffering system does not need CO2 gas for buffering. unsure.gif

Hope for ideas.



As far as I know, HEPES just slows down changes in pH--it's useful to prevent big pH shifts when you are manipulating your CO2 buffered cultures outside of the incubator.