quantify methyl-Cytosine in global genome - methods to quantify methyl-Cytosine in global genome (Feb/08/2005 )
Hi
I am trying to quantify the global DNA methylation in my samples. I know that the methyl-Cytosine is present in them (by bisulphite sequencing and immunochemical analysis), but the capillary electrophoresis didn’t seem to be enough sensitive to detect it (the detection limit of CE method is at 0.1-0.2 % cytosine methylation). Do you know a more sensitive method (than the capillary electrophoresis) to quantify methyl-Cytosine that it is so confident?
Any advice you can offer would be MUCH appreciated.
Thank you!!!
-ant-
QUOTE (ant @ Feb 8 2005, 10:08 AM)
Hi
I am trying to quantify the global DNA methylation in my samples. I know that the methyl-Cytosine is present in them (by bisulphite sequencing and immunochemical analysis), but the capillary electrophoresis didn’t seem to be enough sensitive to detect it (the detection limit of CE method is at 0.1-0.2 % cytosine methylation). Do you know a more sensitive method (than the capillary electrophoresis) to quantify methyl-Cytosine that it is so confident?
Any advice you can offer would be MUCH appreciated.
Thank you!!!
I am trying to quantify the global DNA methylation in my samples. I know that the methyl-Cytosine is present in them (by bisulphite sequencing and immunochemical analysis), but the capillary electrophoresis didn’t seem to be enough sensitive to detect it (the detection limit of CE method is at 0.1-0.2 % cytosine methylation). Do you know a more sensitive method (than the capillary electrophoresis) to quantify methyl-Cytosine that it is so confident?
Any advice you can offer would be MUCH appreciated.
Thank you!!!
I know HPCL can quantify global methylaition and this method is very sensitive.However ,I know nothing about the detailed protocol of it
-Rovy-