Biocompatibility suggestions - (Dec/27/2004 )

i'm now targetting on this cytokine namely IL-1beta,IL-6,IL-8 and TNF-alpha as these r proinflammatory cytokines. do u think that is this concise enough to survey on only these 4 cytokines where in fact there r other cytokines as well can be included. meanwhile, this research in cytokine assay must be wisely finished within 6 months, do u think is that ok?

Thanks and regrds: sycay

I think the cytokines you pick should be key factors in mediating immunoresponse/biocompatibility and relevant to the later in vivo study (otherwise you won't see the effect of your materials). Second, where are the major source for these inflammatory molecules - keratinocytes or other cell types in skin microenvoronment???

These kind of consideration will help you to decide which cytokine/chemokine to target and which cell type as your primary model system. Once you decide the key factors to target, 6 months is reasonal time line.

from my thought was that these biomaterials will raise up some negative compatibilities on human skin, and thus skin will produce some reaction back to this through chemokines and cytokines as well. i think only interleukin-1 alpha, TNF-alpha would be the major molecules to be surveyed. my initial investigation definitely only on human epidermal keratinocyte. OR do u have any good ideas? just mention, would be well appreciated!! :-)

oh,ya..have u ever plated keratinocyte culture with different cell densities?
what would ur best suggestion on the cell density for the best growth??
currently, i used 10 6 in plating cells, less cell densities seems to inhibit growth as i hav done during preliminary test..it wasnt a simple work due to i didnt culture using feeder layer...althoguth some books suggested that serum free media with bovine pituitary extract and epidermal growth factors would support growth without feeder layer for keratinocyte culture..it didnt show a good review at last. collagen matrix would be used instead of feeder layer, from what i thought! what u think??

dear post doc,

do u hav e any ideas to further culture the monolayer keratinocyte to be multilayer as i wanna use it as allogenic graft for my patient. i heard that the simple way is the additional concentration of calcium and magnesium to further its differentiation process...but i still wanna find it in detail protocol.

thanks for ur ideas!