Enzyme inhibition - (Jan/07/2009 )
IS this general Technical approach for enzyme inhibition correct???
i need opinions. THANX
General Technical approach for enzyme inhibition:
1. Extraction for the enzyme from the cell.
2. Purification of the enzyme.
3. Determine the activity of enzyme before inhibition.
4. Applying the inhibition mechanism to the enzyme (and determination of proper inhibition condition such as pH, temperature and concentration of the inhibitor.)
5. Determine the activity of enzyme after the inhibition.
6. The influence of several parameters such as: a) pH and temperature on the enzyme activity. 
Kinetic parameter, the Michael's constant KM and VMax for the enzyme before and after the inhibition.
7. The sets used: Rats, pH measurements, Spectrophotometric measurements, microscopic observation, chromatographic measurements, x-ray diffraction.
i need opinions. THANX
General Technical approach for enzyme inhibition:
1. Extraction for the enzyme from the cell.
2. Purification of the enzyme.
3. Determine the activity of enzyme before inhibition.
4. Applying the inhibition mechanism to the enzyme (and determination of proper inhibition condition such as pH, temperature and concentration of the inhibitor.)
5. Determine the activity of enzyme after the inhibition.
6. The influence of several parameters such as: a) pH and temperature on the enzyme activity.
Kinetic parameter, the Michael's constant KM and VMax for the enzyme before and after the inhibition. 7. The sets used: Rats, pH measurements, Spectrophotometric measurements, microscopic observation, chromatographic measurements, x-ray diffraction.
nice strategy, however, much work, especially point 4. to reveal the mechanism which needs crystallization or at least simulation studies will be hard work...