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how to get rid of the agrose from sweater - (Dec/06/2008 )

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hello everyone,
i wander if you have the same experience that when you boil the agrose in a microwave oven, and when you get the bottle out to shake it. And the agrose in it spill out by accident, then your sweater is speckled. That was what happened to me, i don't know how to get rid of it. And the sweater is what i loved so much, i don't think i have to throw it away.

-yiruz-

can you wash it with hot water?

if not, put a unwanted cloth on the area that have agarose and use a warm iron to melt the agarose
Then use a cloth soaked with warm water to remove the agarose.

hope this may help.

-Minnie Mouse-

QUOTE (yiruz @ Dec 7 2008, 06:00 AM)
hello everyone,
i wander if you have the same experience that when you boil the agrose in a microwave oven, and when you get the bottle out to shake it. And the agrose in it spill out by accident, then your sweater is speckled. That was what happened to me, i don't know how to get rid of it. And the sweater is what i loved so much, i don't think i have to throw it away.


use agarase - if you really love it. wink.gif
Anyway a normal machine wash should do it, too (?).

Hopefully no EtBr already inside the gel, then I'd throw it away.

-hobglobin-

QUOTE (Minnie Mouse @ Dec 7 2008, 03:02 PM)
can you wash it with hot water?

if not, put a unwanted cloth on the area that have agarose and use a warm iron to melt the agarose
Then use a cloth soaked with warm water to remove the agarose.

hope this may help.


thank you! i will have a try.

-yiruz-


thank you for your reply.

i haven't add EtBr into the gel this time, but the triangle bottle holded the gel had been used to fill the gel added EtBr before.

Should i really need to throw my lovely sweater away?

-yiruz-

Should depend on the amount of EtBr that was there. Putting it out in the sun should help get rid of some.

But aren't you supposed to be wearing a lab-coat when inside you lab?

-Bungalow Boy-

QUOTE (Bungalow Boy @ Dec 7 2008, 11:18 PM)
Should depend on the amount of EtBr that was there. Putting it out in the sun should help get rid of some.

But aren't you supposed to be wearing a lab-coat when inside you lab?


No. I always wear a lab-coat in my lab, but sometimes i am in a hurry and forget wearing it, then things happen. I repent of my careless very much now.

Do you mean the sunshine may help to get rid of EtBr in agrose?

-yiruz-

Yes. U can check under UV if EtBr is still there.

-Bungalow Boy-

QUOTE (Bungalow Boy @ Dec 8 2008, 12:06 AM)
Yes. U can check under UV if EtBr is still there.


Good idea, thank you!

-yiruz-

QUOTE (yiruz @ Dec 7 2008, 11:40 PM)
QUOTE (Bungalow Boy @ Dec 8 2008, 12:06 AM)
Yes. U can check under UV if EtBr is still there.


Good idea, thank you!


At first, i thought it was a good idea. But now i don't think so, see below:

Ethidium bromide is a large, flat basic molecule that resembles a DNA base pair. Because of its chemical structure, it
can intercalate (or insert) into a DNA strand. Ethidium bromide is commonly used in molecular biology laboratories to
stain electrophoresis gels. The compound forms fluorescent complexes with nucleic acids and these can be viewed
under UV light.


Only after the EtBr and nucleic acid forming complexes, you can view the compound under UV light.

-yiruz-

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