Protocol Online logo
Top : Forum Archives: : Biochemistry

screening for enzyme activity - (Nov/18/2008 )

hi there
may I knw what is the correct protocol to screen enzyme activity? I read a paper which they did screening for enzyme activity. but they didnt provide a proper method. medium they used was R2A agar plates supplmented with starch, skim milk, lactose etc.. I m looking for beta galactosidase, xylanase, chitinase and keratinase. can anyone help me with the right procedure? i need to know how to prepare media, inoculation, and how to know if there is enzyme activity? thanks in avance.

Abby05

-Abby05-

The paper you read probably has a reference to a method. Also, try the Advances in Enzyme Regulation or the Advances in Enzymology series. You'll get them online.

-paraboxa-

QUOTE (Abby05 @ Nov 18 2008, 02:06 AM)
hi there
may I knw what is the correct protocol to screen enzyme activity? I read a paper which they did screening for enzyme activity. but they didnt provide a proper method. medium they used was R2A agar plates supplmented with starch, skim milk, lactose etc.. I m looking for beta galactosidase, xylanase, chitinase and keratinase. can anyone help me with the right procedure? i need to know how to prepare media, inoculation, and how to know if there is enzyme activity? thanks in avance.

Abby05


try to contact the authors

-The Bearer-

Thanks buddies..

-Abby05-