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very important - electrophoresis problem (Nov/16/2008 )

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QUOTE (dr.dalia @ Nov 28 2008, 07:11 PM)
bands faint quickly before the marker separates well
so that to get good obvious bands, marker is not well separated yet & needs more time,
but if i left it for more time, bands faints !!

I don't know how to fix that. Can the problem be the agar type or what???

most likely caused by migration of ethidium bromide. it migrates through the gel in the opposite direction that the nucleic acids migrate.

you can include etbr in the electrode buffer or stain after running the gel.


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