Searching for an alternative Housekeeping protein - tried GAPDH and beta-actin (Nov/14/2008 )
hallo,
in my mouse samples both housekeeping proteins are regulated. does any of you recommend an alternative antibody to use? i also stained transfered proteins via amidoblack staining on membrane to get a view of protein amounts, donīt know how sensitive this is!
thanks for suggestions
-moljul-
QUOTE (moljul @ Nov 14 2008, 02:53 AM)
hallo,
in my mouse samples both housekeeping proteins are regulated. does any of you recommend an alternative antibody to use? i also stained transfered proteins via amidoblack staining on membrane to get a view of protein amounts, donīt know how sensitive this is!
thanks for suggestions
in my mouse samples both housekeeping proteins are regulated. does any of you recommend an alternative antibody to use? i also stained transfered proteins via amidoblack staining on membrane to get a view of protein amounts, donīt know how sensitive this is!
thanks for suggestions
you can try lamin, histone H2b, beta tubulin etc.
-cellcounter-